Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 443341..443869 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EKN05_RS02315 Protein ID WP_005061113.1
Coordinates 443341..443571 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag EKN05_RS02320 Protein ID WP_000147081.1
Coordinates 443561..443869 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS02280 (EKN05_002290) 438409..438609 - 201 WP_000111769.1 protein ninH -
EKN05_RS02285 (EKN05_002295) 438704..438961 - 258 WP_001064799.1 hypothetical protein -
EKN05_RS02290 (EKN05_002300) 438958..440349 - 1392 WP_000211321.1 replicative DNA helicase -
EKN05_RS02295 (EKN05_002305) 440346..441224 - 879 WP_000988183.1 ATP-binding protein -
EKN05_RS02300 (EKN05_002310) 441235..442143 - 909 WP_001247829.1 hypothetical protein -
EKN05_RS02305 (EKN05_002315) 442130..442363 - 234 WP_000618003.1 hypothetical protein -
EKN05_RS25530 442360..442812 - 453 WP_232626950.1 Rha family transcriptional regulator -
EKN05_RS25535 442870..443328 - 459 WP_000961309.1 ash family protein -
EKN05_RS02315 (EKN05_002325) 443341..443571 + 231 WP_005061113.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKN05_RS02320 (EKN05_002330) 443561..443869 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
EKN05_RS02325 (EKN05_002335) 443911..444087 - 177 WP_024260406.1 NinE family protein -
EKN05_RS02330 (EKN05_002340) 444084..444494 - 411 WP_000814621.1 recombination protein NinB -
EKN05_RS02335 (EKN05_002345) 444491..444769 - 279 WP_005061104.1 hypothetical protein -
EKN05_RS02340 (EKN05_002350) 444844..447750 - 2907 WP_005105712.1 toprim domain-containing protein -
EKN05_RS02345 (EKN05_002355) 447858..447974 - 117 Protein_450 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 407819..456347 48528


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(5-70)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8665.12 Da        Isoelectric Point: 10.5308

>T120361 WP_005061113.1 NZ_CP037923:443341-443571 [Shigella flexneri 5a str. M90T]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW

Download         Length: 231 bp

>T120361 NZ_CP037923:443341-443571 [Shigella flexneri 5a str. M90T]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT120361 WP_000147081.1 NZ_CP037923:443561-443869 [Shigella flexneri 5a str. M90T]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT120361 NZ_CP037923:443561-443869 [Shigella flexneri 5a str. M90T]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References