Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 941327..941894 Replicon chromosome
Accession NZ_CP026799
Organism Shigella flexneri strain NCTC 9728

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag C1P82_RS04880 Protein ID WP_130862220.1
Coordinates 941327..941596 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C1P82_RS04885 Protein ID WP_000147081.1
Coordinates 941586..941894 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P82_RS04845 936434..936634 - 201 WP_000111769.1 protein ninH -
C1P82_RS04850 936729..936986 - 258 WP_001064799.1 hypothetical protein -
C1P82_RS04855 936983..938374 - 1392 WP_000211321.1 replicative DNA helicase -
C1P82_RS04860 938371..939249 - 879 WP_000988183.1 ATP-binding protein -
C1P82_RS04865 939260..940168 - 909 WP_001247829.1 hypothetical protein -
C1P82_RS04870 940155..940388 - 234 WP_000618004.1 hypothetical protein -
C1P82_RS04875 940385..941401 - 1017 Protein_931 Rha family transcriptional regulator -
C1P82_RS04880 941327..941596 + 270 WP_130862220.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P82_RS04885 941586..941894 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C1P82_RS04890 941936..942112 - 177 WP_024260406.1 NinE family protein -
C1P82_RS04895 942109..942519 - 411 WP_000814621.1 recombination protein NinB -
C1P82_RS04900 942516..942794 - 279 WP_005061104.1 hypothetical protein -
C1P82_RS04905 942869..945775 - 2907 WP_134807586.1 toprim domain-containing protein -
C1P82_RS24080 945883..945999 - 117 Protein_938 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 905846..954372 48526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(4-83)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10324.08 Da        Isoelectric Point: 10.7080

>T96017 WP_130862220.1 NZ_CP026799:941327-941596 [Shigella flexneri]
MSMRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAET
RMKEVKHDW

Download         Length: 270 bp

>T96017 NZ_CP026799:941327-941596 [Shigella flexneri]
TTGAGTATGCGTGCCAGATACCAGCATCTTGTTGAACGCATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAG
CGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAG
GGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAGCGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACC
AGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT96017 WP_000147081.1 NZ_CP026799:941586-941894 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT96017 NZ_CP026799:941586-941894 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References