Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2767337..2767898 Replicon chromosome
Accession NC_008258
Organism Shigella flexneri 5 str. 8401

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag SFV_RS26075 Protein ID WP_001202685.1
Coordinates 2767635..2767898 (-) Length 88 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag SFV_RS14960 Protein ID WP_000147081.1
Coordinates 2767337..2767645 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFV_RS27540 2763234..2763350 + 117 Protein_2728 helix-turn-helix domain-containing protein -
SFV_RS14940 2763458..2766364 + 2907 WP_005105712.1 toprim domain-containing protein -
SFV_RS14945 2766527..2766715 + 189 WP_005105713.1 hypothetical protein -
SFV_RS14950 2766712..2767122 + 411 WP_000814621.1 recombination protein NinB -
SFV_RS14955 2767119..2767295 + 177 WP_024260406.1 NinE family protein -
SFV_RS14960 2767337..2767645 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
SFV_RS26075 2767635..2767898 - 264 WP_001202685.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SFV_RS14965 2767830..2768336 + 507 WP_044024397.1 ash family protein -
SFV_RS24525 2768352..2768846 + 495 WP_094096580.1 Rha family transcriptional regulator -
SFV_RS14975 2768843..2769076 + 234 WP_000618003.1 hypothetical protein -
SFV_RS14980 2769063..2769971 + 909 WP_001247829.1 hypothetical protein -
SFV_RS14985 2769982..2770860 + 879 WP_000988183.1 ATP-binding protein -
SFV_RS14990 2770857..2772248 + 1392 WP_000211321.1 replicative DNA helicase -
SFV_RS14995 2772245..2772502 + 258 WP_001064799.1 hypothetical protein -
SFV_RS15000 2772595..2772795 + 201 WP_000111769.1 protein ninH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2754863..2803389 48526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(2-81)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10105.81 Da        Isoelectric Point: 10.7080

>T21664 WP_001202685.1 NC_008258:c2767898-2767635 [Shigella flexneri 5 str. 8401]
MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRM
KEVKHDW

Download         Length: 264 bp

>T21664 NC_008258:c2767898-2767635 [Shigella flexneri 5 str. 8401]
ATGCGTGCCAGATACCAGCATCTTGTTGAACGCATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTT
TGGTGACGGGCTTTTCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAAC
GCATCATCATGCTGCATAGTTTTGTAAAGAAAGCGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATG
AAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT21664 WP_000147081.1 NC_008258:c2767645-2767337 [Shigella flexneri 5 str. 8401]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT21664 NC_008258:c2767645-2767337 [Shigella flexneri 5 str. 8401]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References