Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) PanAT/-
Location 1866048..1867735 Replicon chromosome
Accession NZ_CP014038
Organism Vibrio harveyi strain FDAARGOS_107

Toxin (Protein)


Gene name panT Uniprot ID A0A454B6K5
Locus tag AL538_RS09490 Protein ID WP_061065447.1
Coordinates 1866048..1867271 (-) Length 408 a.a.

Antitoxin (Protein)


Gene name panA Uniprot ID A0A454B6J0
Locus tag AL538_RS09495 Protein ID WP_061065448.1
Coordinates 1867268..1867735 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL538_RS09475 (AL538_08815) 1861685..1862368 - 684 WP_050937483.1 ATPase -
AL538_RS09480 (AL538_08820) 1862451..1864304 - 1854 WP_061065446.1 TonB-dependent receptor -
AL538_RS09485 (AL538_08825) 1864748..1865857 + 1110 WP_017187950.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
AL538_RS09490 (AL538_08830) 1866048..1867271 - 1224 WP_061065447.1 RelA/SpoT domain-containing protein Toxin
AL538_RS09495 (AL538_08835) 1867268..1867735 - 468 WP_061065448.1 DUF4065 domain-containing protein Antitoxin
AL538_RS09500 (AL538_08840) 1867991..1868662 - 672 WP_061065449.1 hypothetical protein -
AL538_RS09505 (AL538_08845) 1868807..1869841 + 1035 WP_061065450.1 hypothetical protein -
AL538_RS09510 (AL538_08850) 1870008..1870652 + 645 WP_061065451.1 inovirus Gp2 family protein -
AL538_RS09515 (AL538_08855) 1870693..1871139 - 447 WP_061065452.1 hypothetical protein -
AL538_RS09520 (AL538_08860) 1871250..1872239 - 990 WP_061065453.1 50S ribosome-binding GTPase -
AL538_RS09525 (AL538_08865) 1872317..1872538 + 222 WP_061065454.1 AlpA family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RelA/SpoT

(86-226)

Antitoxin

Antitoxin SocA-like, Panacea domain

(32-123)


Sequences

Toxin        


Download         Length: 408 a.a.        Molecular weight: 45891.67 Da        Isoelectric Point: 5.5470

>T10013 WP_061065447.1 NZ_CP014038:c1867271-1866048 [Vibrio harveyi]
VSNKENSVVSFYIDDTPTAEQASKTFQDDYELNFQDFIERTVNESAAEDFSYSNNGVKKAGRRLRKREGDLKSATATIQQ
FRAAHEKPLNTIAYLVGRCCRELGIDVKPVTRLKRLETIVDKLQRKSLDGETANATCVTNMNDIGGCRAIFPDLISLEKV
KSQLQLTVEKVGRVRIKDIDDYITSPKPNDCGYRSLHVIYRYDHASGKSFNIEAQLRTRLQHLWATTVEIVDILEGTKIK
THSHSSNEDKSSLQVQWEELLSIMSQYIADAEGAIELSDIDKANFSKRLVVLDREINALNRLSSFKILSEKVNSCCDSSI
EHVLLVINETSRELIFSEEFENYSQAISVYNAAEKVTRSYSRSTSRLNTLLISTKNMGQLSEAYPNYLGDCASFITLLED
AMLSGES

Download         Length: 1224 bp

>T10013 NZ_CP014038:c1867271-1866048 [Vibrio harveyi]
GTGAGTAACAAAGAAAATAGTGTCGTCAGTTTTTACATTGACGACACCCCCACAGCTGAACAAGCTTCAAAAACCTTCCA
AGACGACTACGAACTGAACTTTCAAGATTTTATAGAAAGAACGGTAAATGAAAGTGCTGCTGAAGACTTCTCGTACTCTA
ATAATGGAGTAAAGAAAGCAGGAAGACGGCTTCGTAAGAGAGAGGGCGACCTCAAGTCAGCTACTGCTACAATCCAACAG
TTTCGAGCCGCACACGAAAAGCCATTAAACACTATCGCATATCTTGTTGGTCGCTGTTGCCGTGAGCTGGGTATAGATGT
TAAACCAGTTACACGGTTAAAGCGCTTAGAAACAATCGTAGATAAGTTGCAGCGTAAATCTCTTGATGGTGAAACGGCAA
ATGCTACATGTGTGACCAATATGAATGATATTGGCGGCTGCAGGGCGATTTTTCCGGATTTAATTTCATTGGAGAAGGTG
AAATCTCAACTGCAACTTACAGTTGAGAAGGTAGGCCGAGTTAGAATTAAAGATATTGATGACTATATCACTAGCCCTAA
GCCTAATGATTGTGGTTACCGAAGCTTGCATGTGATTTATCGCTATGACCATGCTAGTGGTAAAAGTTTTAACATTGAGG
CTCAGCTACGTACTCGGCTTCAACATCTTTGGGCTACTACTGTGGAAATTGTCGACATCCTGGAAGGTACCAAGATTAAG
ACTCACTCTCACAGTTCAAATGAAGACAAGAGCAGTTTACAGGTTCAATGGGAAGAATTGCTTTCGATAATGAGCCAGTA
CATTGCTGATGCTGAAGGGGCTATAGAGCTAAGTGATATCGATAAAGCCAATTTTTCAAAGCGCTTGGTCGTCTTGGACC
GCGAAATCAATGCGCTGAATCGGTTGAGTAGTTTTAAAATTTTATCTGAAAAGGTGAATTCTTGTTGTGATAGTTCCATT
GAGCATGTATTGCTAGTTATTAACGAAACTTCACGAGAATTGATCTTTAGTGAAGAGTTTGAAAATTACAGCCAAGCAAT
TTCAGTTTATAACGCAGCTGAGAAAGTCACTCGTAGTTACTCAAGATCAACCTCGAGGCTCAATACCCTTCTCATATCCA
CAAAAAATATGGGCCAACTTTCGGAGGCTTACCCTAACTATCTAGGCGATTGTGCCTCATTCATCACTCTGCTTGAAGAC
GCAATGCTTTCTGGTGAAAGTTAA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17512.98 Da        Isoelectric Point: 7.9061

>AT10013 WP_061065448.1 NZ_CP014038:c1867735-1867268 [Vibrio harveyi]
MKAYHTYSAVDVAWSLLKHAKEQGKCFSNLQLQKLTYVCHGLSLAHFQRPLVVDDVFAWKFGPVVPSVYFRFKSYGSSVI
PEQCDVALDQESESIVKDVVSKLGHLTGPQLVDLTHREGSPWHQVWDGTHQKVIPDHLIQAHYTQIKQSGRTASL

Download         Length: 468 bp

>AT10013 NZ_CP014038:c1867735-1867268 [Vibrio harveyi]
ATGAAAGCATATCACACATACAGTGCTGTAGACGTTGCATGGAGCTTGCTCAAGCATGCGAAAGAGCAAGGTAAATGCTT
CTCCAATTTGCAGCTACAAAAACTTACCTATGTTTGTCATGGTTTATCATTGGCGCATTTCCAGCGTCCTCTCGTCGTAG
ATGATGTATTTGCTTGGAAGTTTGGTCCAGTGGTGCCATCTGTTTACTTCCGCTTTAAATCTTACGGTAGTAGTGTGATC
CCTGAACAATGTGATGTAGCACTAGACCAAGAAAGTGAGTCTATTGTCAAAGATGTAGTCTCTAAGCTGGGTCATTTGAC
GGGGCCTCAACTTGTAGATTTAACTCACCGCGAAGGAAGCCCATGGCATCAGGTATGGGATGGCACACACCAAAAAGTGA
TTCCGGACCATTTGATTCAAGCCCATTACACTCAAATCAAACAATCCGGACGAACAGCTAGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT10008 Helicobacter sp. 13S00482-2 NODE_7_length_88364_cov_126.498_ID_13

37.589

96.575

0.363
AT10007 Escherichia coli STEC_O31

37.063

92.258

0.342
AT10009 Bartonella apis strain BBC0122

36.111

92.903

0.335
AT10010 Burkholderia prophage phi52237

31.579

98.065

0.31
AT6367 Caulobacter crescentus NA1000

34.307

88.387

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A454B6K5

Antitoxin

Source ID Structure
AlphaFold DB A0A454B6J0

References

(1) Tatsuaki Kurata et al. (2022) A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains. Proceedings of The National Academy of Sciences of The United States of America 119(6):e2102212119. [PubMed:35121656] experimental


experimental experimental literature