Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PanAT/- |
| Location | 2348..3590 | Replicon | chromosome |
| Accession | NZ_MLAT01000007 | ||
| Organism | Helicobacter sp. 13S00482-2 NODE_7_length_88364_cov_126.498_ID_13 | ||
Toxin (Protein)
| Gene name | panT | Uniprot ID | - |
| Locus tag | BKH42_RS04665 | Protein ID | WP_095295403.1 |
| Coordinates | 2778..3590 (+) | Length | 271 a.a. |
Antitoxin (Protein)
| Gene name | panA | Uniprot ID | - |
| Locus tag | BKH42_RS04660 | Protein ID | WP_095295401.1 |
| Coordinates | 2348..2788 (+) | Length | 147 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BKH42_RS04650 (BKH42_04650) | 1..658 | - | 658 | WP_143428549.1 | hypothetical protein | - |
| BKH42_RS09475 | 1109..1279 | + | 171 | WP_180673977.1 | hypothetical protein | - |
| BKH42_RS04655 (BKH42_04655) | 1333..2139 | + | 807 | WP_095295399.1 | hypothetical protein | - |
| BKH42_RS04660 (BKH42_04660) | 2348..2788 | + | 441 | WP_095295401.1 | DUF4065 domain-containing protein | Antitoxin |
| BKH42_RS04665 (BKH42_04665) | 2778..3590 | + | 813 | WP_095295403.1 | hypothetical protein | Toxin |
| BKH42_RS04670 (BKH42_04670) | 3677..4123 | - | 447 | WP_180673978.1 | ImmA/IrrE family metallo-endopeptidase | - |
| BKH42_RS09480 | 4442..4612 | - | 171 | WP_180673979.1 | hypothetical protein | - |
| BKH42_RS04680 (BKH42_04680) | 5428..6126 | - | 699 | WP_095295407.1 | lysophospholipid acyltransferase family protein | - |
| BKH42_RS04685 (BKH42_04685) | 6113..7237 | - | 1125 | WP_095295409.1 | GW dipeptide domain-containing protein | - |
| BKH42_RS04690 (BKH42_04690) | 7234..7896 | - | 663 | WP_095295411.1 | phosphoribosylformylglycinamidine synthase subunit PurQ | - |
| BKH42_RS04695 (BKH42_04695) | 7900..8145 | - | 246 | WP_095295413.1 | phosphoribosylformylglycinamidine synthase subunit PurS | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 271 a.a. Molecular weight: 31819.55 Da Isoelectric Point: 6.3471
>T10008 WP_095295403.1 NZ_MLAT01000007:2778-3590 [Helicobacter sp. 13S00482-2]
MDTNKKILVFLKRISTEEFDTDDKYQRAKEEIRKIYSDRDFRHHYSDITAQILKSADLEKLVENLRIVKEGIEEDKDEDS
KHYLHQLTKLWDHINLEFVRVGYYKDRFSDIENRLSDINKSIENTQNGLQNLQDNNKIIQTNIKDAQIQLQNLKKDSEDI
QASIRNQQNQYITILGIFASIVLTLVGGFIFSTSVFANIDKSSIYRLVFMMIFICCFTTNILYYLFRFIKNIGEKNPDCI
FKDKAIVSFNILMLFILICDGGFYLFLSYC
MDTNKKILVFLKRISTEEFDTDDKYQRAKEEIRKIYSDRDFRHHYSDITAQILKSADLEKLVENLRIVKEGIEEDKDEDS
KHYLHQLTKLWDHINLEFVRVGYYKDRFSDIENRLSDINKSIENTQNGLQNLQDNNKIIQTNIKDAQIQLQNLKKDSEDI
QASIRNQQNQYITILGIFASIVLTLVGGFIFSTSVFANIDKSSIYRLVFMMIFICCFTTNILYYLFRFIKNIGEKNPDCI
FKDKAIVSFNILMLFILICDGGFYLFLSYC
Download Length: 813 bp
>T10008 NZ_MLAT01000007:2778-3590 [Helicobacter sp. 13S00482-2]
TTGGATACTAATAAGAAAATTTTAGTTTTTTTAAAAAGAATTTCTACAGAAGAATTTGATACTGATGATAAATATCAAAG
AGCTAAGGAAGAGATCAGAAAAATATACTCTGATAGAGATTTTAGGCATCATTATTCTGATATCACAGCCCAGATTTTAA
AAAGTGCTGATCTAGAAAAACTAGTCGAAAATCTTAGAATTGTTAAGGAGGGAATAGAAGAAGATAAAGATGAAGATAGC
AAACACTATCTTCATCAGCTGACTAAGCTATGGGATCATATCAACCTTGAATTCGTTCGTGTAGGATATTATAAAGATCG
TTTCTCAGATATAGAAAATCGTTTATCAGATATAAACAAGTCCATAGAAAACACCCAAAACGGACTTCAAAACCTACAAG
ACAACAATAAGATTATTCAAACAAACATAAAAGATGCTCAAATCCAACTTCAAAACTTAAAAAAAGATAGCGAGGATATT
CAAGCAAGTATCAGAAATCAACAAAACCAATACATCACCATCTTAGGAATCTTTGCCTCGATTGTTTTAACCCTTGTGGG
TGGTTTTATCTTTTCAACCTCCGTGTTTGCGAACATCGATAAAAGCAGTATCTATCGACTGGTGTTTATGATGATCTTCA
TTTGTTGTTTTACCACGAATATTCTTTATTATCTCTTTCGCTTTATCAAAAATATAGGCGAGAAGAACCCTGATTGTATC
TTTAAAGATAAGGCTATTGTCAGCTTTAATATATTGATGCTTTTTATATTGATATGCGATGGGGGTTTTTATCTTTTTTT
ATCTTATTGTTAG
TTGGATACTAATAAGAAAATTTTAGTTTTTTTAAAAAGAATTTCTACAGAAGAATTTGATACTGATGATAAATATCAAAG
AGCTAAGGAAGAGATCAGAAAAATATACTCTGATAGAGATTTTAGGCATCATTATTCTGATATCACAGCCCAGATTTTAA
AAAGTGCTGATCTAGAAAAACTAGTCGAAAATCTTAGAATTGTTAAGGAGGGAATAGAAGAAGATAAAGATGAAGATAGC
AAACACTATCTTCATCAGCTGACTAAGCTATGGGATCATATCAACCTTGAATTCGTTCGTGTAGGATATTATAAAGATCG
TTTCTCAGATATAGAAAATCGTTTATCAGATATAAACAAGTCCATAGAAAACACCCAAAACGGACTTCAAAACCTACAAG
ACAACAATAAGATTATTCAAACAAACATAAAAGATGCTCAAATCCAACTTCAAAACTTAAAAAAAGATAGCGAGGATATT
CAAGCAAGTATCAGAAATCAACAAAACCAATACATCACCATCTTAGGAATCTTTGCCTCGATTGTTTTAACCCTTGTGGG
TGGTTTTATCTTTTCAACCTCCGTGTTTGCGAACATCGATAAAAGCAGTATCTATCGACTGGTGTTTATGATGATCTTCA
TTTGTTGTTTTACCACGAATATTCTTTATTATCTCTTTCGCTTTATCAAAAATATAGGCGAGAAGAACCCTGATTGTATC
TTTAAAGATAAGGCTATTGTCAGCTTTAATATATTGATGCTTTTTATATTGATATGCGATGGGGGTTTTTATCTTTTTTT
ATCTTATTGTTAG
Antitoxin
Download Length: 147 a.a. Molecular weight: 17131.74 Da Isoelectric Point: 6.1068
>AT10008 WP_095295401.1 NZ_MLAT01000007:2348-2788 [Helicobacter sp. 13S00482-2]
MKALDVAKYIINKCIEKGKPISNLQLQKITYFVHLGFLRETHRKLIDDESFKAWQYGPVIKSVYNKFRIYGGFTIDDIQE
CHIELKPEEEKSIDKILDECIKLKPWELVQRSHNPDGAWIKVYKDGEDYKDIAIPDNLIQEEALGY
MKALDVAKYIINKCIEKGKPISNLQLQKITYFVHLGFLRETHRKLIDDESFKAWQYGPVIKSVYNKFRIYGGFTIDDIQE
CHIELKPEEEKSIDKILDECIKLKPWELVQRSHNPDGAWIKVYKDGEDYKDIAIPDNLIQEEALGY
Download Length: 441 bp
>AT10008 NZ_MLAT01000007:2348-2788 [Helicobacter sp. 13S00482-2]
ATGAAAGCATTAGATGTAGCTAAATATATTATCAATAAATGCATAGAAAAGGGCAAACCTATCAGCAATCTTCAGCTCCA
AAAAATTACATATTTTGTTCATTTAGGATTTTTGAGAGAAACCCATAGAAAACTTATCGATGATGAAAGTTTTAAAGCTT
GGCAATATGGACCCGTGATAAAGAGTGTCTATAATAAGTTTAGAATCTATGGTGGATTTACCATTGATGATATCCAAGAA
TGTCATATTGAATTAAAACCCGAAGAAGAAAAGTCAATAGATAAGATTCTAGATGAATGTATCAAGTTGAAACCTTGGGA
ACTTGTCCAAAGAAGCCATAATCCAGATGGTGCGTGGATAAAAGTATACAAAGATGGGGAAGACTATAAAGATATTGCAA
TTCCTGATAATCTAATTCAAGAGGAAGCTCTTGGATACTAA
ATGAAAGCATTAGATGTAGCTAAATATATTATCAATAAATGCATAGAAAAGGGCAAACCTATCAGCAATCTTCAGCTCCA
AAAAATTACATATTTTGTTCATTTAGGATTTTTGAGAGAAACCCATAGAAAACTTATCGATGATGAAAGTTTTAAAGCTT
GGCAATATGGACCCGTGATAAAGAGTGTCTATAATAAGTTTAGAATCTATGGTGGATTTACCATTGATGATATCCAAGAA
TGTCATATTGAATTAAAACCCGAAGAAGAAAAGTCAATAGATAAGATTCTAGATGAATGTATCAAGTTGAAACCTTGGGA
ACTTGTCCAAAGAAGCCATAATCCAGATGGTGCGTGGATAAAAGTATACAAAGATGGGGAAGACTATAAAGATATTGCAA
TTCCTGATAATCTAATTCAAGAGGAAGCTCTTGGATACTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10013 | Vibrio harveyi strain FDAARGOS_107 |
37.589 |
96.575 |
0.363 |
| AT10004 | Bacillus subtilis strain Ia1a |
30 |
100 |
0.329 |
| AT10011 | Bifidobacterium ruminantium strain An807 An807_NODE_4_length_147701_cov_109.271 |
30.201 |
100 |
0.308 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Tatsuaki Kurata et al. (2022) A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains. Proceedings of The National Academy of Sciences of The United States of America 119(6):e2102212119. [PubMed:35121656]