Detailed information of TA system TA010011

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/-
• Location: 115003..115883
• Replicon: chromosome
• Accession: NZ_JACJUA010000004
• Organism: Bifidobacterium ruminantium strain An807 An807_NODE_4_length_147701_cov_109.271

Toxin (Protein)

• Gene name: panT
• Uniprot ID: A0A412K6Y8
• Locus tag: H6G88_RS06330
• Protein ID: WP_026646888.1
• Coordinates: 115560..115883 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: -
• Locus tag: H6G88_RS06325
• Protein ID: WP_081815666.1
• Coordinates: 115003..115563 (+)
• Length: 187 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H6G88_RS06290 (H6G88_06290)	110312..111268	-	957	WP_204442301.1	nucleoside hydrolase	-
H6G88_RS06295 (H6G88_06295)	111503..112552	-	1050	WP_204442302.1	LacI family DNA-binding transcriptional regulator	-
H6G88_RS06300 (H6G88_06300)	112748..113074	+	327	WP_117760095.1	hypothetical protein	-
H6G88_RS06305 (H6G88_06305)	113205..113795	+	591	WP_239459083.1	helix-turn-helix domain-containing protein	-
H6G88_RS06310 (H6G88_06310)	113792..114163	+	372	WP_026646891.1	DUF86 domain-containing protein	-
H6G88_RS06315 (H6G88_06315)	114334..114588	+	255	WP_117760097.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
H6G88_RS06320 (H6G88_06320)	114594..114863	+	270	WP_204442325.1	Txe/YoeB family addiction module toxin	-
H6G88_RS06325 (H6G88_06325)	115003..115563	+	561	WP_081815666.1	DUF4065 domain-containing protein	Antitoxin
H6G88_RS06330 (H6G88_06330)	115560..115883	+	324	WP_026646888.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H6G88_RS06335 (H6G88_06335)	115887..116912	-	1026	WP_204442326.1	dTDP-glucose 4,6-dehydratase	-
H6G88_RS06340 (H6G88_06340)	117134..119710	+	2577	WP_239459093.1	alkaline phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	110312..137801	27489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-101)

Antitoxin

• Antitoxin SocA-like, Panacea domain (33-128)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12703.63 Da
• Isoelectric Point: 8.0065

>T10011 WP_026646888.1 NZ_JACJUA010000004:115560-115883 [Bifidobacterium ruminantium]
MTDVRPWQIWRVWFPFREDPTNGKYRPVVIKEITEDGCVVIYVTSQVGKTNFPDFLLIHNWEEAGLNRASAVRYRRLLKI
PLSALGEYVGELSPYDRIELQMKYQFH

Nucleotide

• Length: 324 bp

>T10011 NZ_JACJUA010000004:115560-115883 [Bifidobacterium ruminantium]
TTGACCGATGTACGTCCATGGCAAATTTGGAGGGTCTGGTTCCCTTTTCGAGAGGATCCGACAAACGGGAAATACCGACC
TGTCGTAATCAAGGAAATCACCGAAGACGGTTGCGTGGTGATTTATGTGACGTCGCAAGTCGGCAAGACCAATTTCCCGG
ACTTCTTGCTTATTCATAATTGGGAAGAAGCCGGACTCAACCGAGCATCCGCGGTTCGATATCGGAGATTACTCAAGATT
CCGTTGAGTGCACTTGGCGAATACGTCGGTGAGCTTTCTCCCTATGATCGCATTGAGCTGCAGATGAAATACCAATTCCA
CTGA

Antitoxin

Protein

• Length: 187 a.a.
• Molecular weight: 21407.17 Da
• Isoelectric Point: 4.4320

>AT10011 WP_081815666.1 NZ_JACJUA010000004:115003-115563 [Bifidobacterium ruminantium]
MALMNVMDVADFFISTANEGDPEEDDGITNMKLNKLLFFAQAASLQRFGKPLFDTPLEAWKYGPVVKDVYRTFKGYQRDS
ITKVAQPFDWQTVQPEVLELLCDVYRTYARDYSATGLMRMTHKPDTPWSKVYQPNQNNEIPCELIDEWVKRSPLHIDKDP
TPDNQVTIAKLDASGHPFLPEGWEED

Nucleotide

• Length: 561 bp

>AT10011 NZ_JACJUA010000004:115003-115563 [Bifidobacterium ruminantium]
ATGGCCTTAATGAACGTTATGGACGTAGCGGACTTCTTCATCTCGACCGCCAACGAAGGCGATCCGGAAGAAGATGACGG
CATCACCAACATGAAACTCAACAAACTGCTGTTTTTCGCGCAAGCGGCAAGCCTGCAACGCTTCGGCAAACCACTCTTCG
ACACACCCCTAGAAGCTTGGAAATACGGTCCTGTCGTAAAAGACGTCTACCGAACATTCAAAGGATACCAACGAGATTCC
ATCACCAAAGTCGCCCAACCATTCGACTGGCAAACCGTCCAGCCCGAAGTGCTGGAACTCCTATGCGACGTATACCGCAC
TTATGCGCGAGACTATTCGGCAACAGGCCTGATGCGTATGACACACAAGCCAGACACCCCATGGTCGAAAGTGTATCAAC
CCAACCAAAACAACGAAATCCCCTGCGAACTCATCGATGAATGGGTGAAGCGCTCGCCATTGCATATCGACAAGGATCCG
ACGCCCGACAATCAGGTGACGATTGCCAAGCTCGATGCATCCGGCCATCCGTTCCTTCCCGAAGGATGGGAAGAAGATTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6259	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	37.374	92.523	0.346

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10008	Helicobacter sp. 13S00482-2 NODE_7_length_88364_cov_126.498_ID_13	30.201	100	0.308

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A412K6Y8

References

(1) Tatsuaki Kurata et al. (2022) A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains. Proceedings of The National Academy of Sciences of The United States of America 119(6):e2102212119. [PubMed:35121656]


 experimental literature