10007

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) PanAT/-
Location 54835..55879 Replicon chromosome
Accession NZ_AFEX01000045
Organism Escherichia coli STEC_O31

Toxin (Protein)


Gene name panT Uniprot ID A0A0K4KFL7
Locus tag BE44_RS02910 Protein ID WP_000019185.1
Coordinates 55331..55879 (+) Length 183 a.a.

Antitoxin (Protein)


Gene name panA Uniprot ID A0A839B6W4
Locus tag BE44_RS0125895 Protein ID WP_000287252.1
Coordinates 54835..55308 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE44_RS02930 (ECSTECO31_4531) 50515..51840 + 1326 WP_001295692.1 MATE family efflux transporter DinF -
BE44_RS02925 (ECSTECO31_4532) 51956..52165 + 210 WP_001030593.1 CsbD family protein -
BE44_RS02920 (ECSTECO31_4533) 52207..52722 - 516 WP_001295691.1 zinc uptake transcriptional repressor Zur -
BE44_RS30320 (ECSTECO31_4534) 53040..53330 + 291 WP_001367977.1 hypothetical protein -
BE44_RS0125890 (ECSTECO31_4535) 53350..54062 + 713 Protein_40 DUF2713 family protein -
BE44_RS27975 (ECSTECO31_4537) 54426..54530 + 105 Protein_41 tRNA-dihydrouridine synthase -
BE44_RS0125895 54835..55308 + 474 WP_000287252.1 DUF4065 domain-containing protein Antitoxin
BE44_RS02910 (ECSTECO31_4540) 55331..55879 + 549 WP_000019185.1 hypothetical protein Toxin
BE44_RS02905 (ECSTECO31_4541) 55913..56185 - 273 WP_001093911.1 pyocin activator PrtN family protein -
BE44_RS02900 (ECSTECO31_4542) 56222..56794 - 573 WP_001061345.1 3'-5' exonuclease -
BE44_RS30165 56794..56985 - 192 WP_001690200.1 DUF551 domain-containing protein -
BE44_RS30170 57232..57528 - 297 Protein_47 DUF551 domain-containing protein -
BE44_RS0125900 (ECSTECO31_4544) 57531..57722 - 192 WP_001014290.1 hypothetical protein -
BE44_RS02890 (ECSTECO31_4545) 57724..58278 - 555 WP_001289980.1 ead/Ea22-like family protein -
BE44_RS02885 (ECSTECO31_4546) 58275..58514 - 240 WP_000476212.1 hypothetical protein -
BE44_RS02880 (ECSTECO31_4547) 58507..58710 - 204 WP_000111288.1 hypothetical protein -
BE44_RS02875 (ECSTECO31_4548) 58707..59069 - 363 WP_001242733.1 phage protein -
BE44_RS02870 (ECSTECO31_4549) 59060..59539 - 480 WP_001184458.1 5'-deoxynucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 49834..59443 9609


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Antitoxin SocA-like, Panacea domain

(27-123)


Sequences

Toxin        


Download         Length: 183 a.a.        Molecular weight: 19920.31 Da        Isoelectric Point: 4.5029

>T10007 WP_000019185.1 NZ_AFEX01000045:55331-55879 [Escherichia coli STEC_O31]
MSHNSDIYKLIGAAAGVENGRSEASLNSTDSQEQAFESAFEPSNHERDDDSSSENKAILEAEEFGSNTGALHEFMQQNRM
DSLQAQLDMLKSQVRDKIADATGKEIDNELRTKMASFTVWFMSCWCLFVAGMFVSFLIAHEGKPPVEAIVALLGTSTISI
VGLVGFVVSGLFKSRKDGDKEK

Download         Length: 549 bp

>T10007 NZ_AFEX01000045:55331-55879 [Escherichia coli STEC_O31]
ATGTCTCATAATTCAGATATCTATAAACTGATCGGAGCGGCCGCAGGTGTTGAAAATGGTCGCTCTGAAGCATCCTTAAA
CTCTACTGACAGTCAAGAGCAGGCTTTTGAATCCGCATTTGAGCCGTCTAATCATGAGCGCGATGATGATTCTTCTTCAG
AAAATAAGGCTATTCTGGAGGCAGAAGAGTTTGGTTCTAATACTGGTGCATTGCATGAGTTTATGCAGCAGAACAGAATG
GACAGTCTTCAAGCTCAGCTTGATATGCTCAAGTCACAAGTACGGGATAAGATAGCTGACGCAACTGGTAAAGAAATAGA
CAATGAGCTTCGGACAAAAATGGCCTCATTCACGGTTTGGTTTATGTCGTGTTGGTGCTTATTTGTAGCCGGAATGTTTG
TATCATTTCTTATTGCTCATGAAGGAAAGCCGCCAGTTGAAGCAATCGTTGCATTACTGGGAACAAGTACAATTAGTATT
GTTGGTTTGGTTGGCTTCGTTGTTAGTGGATTGTTCAAATCTAGAAAAGATGGCGATAAAGAAAAATAA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17298.80 Da        Isoelectric Point: 8.4143

>AT10007 WP_000287252.1 NZ_AFEX01000045:54835-55308 [Escherichia coli STEC_O31]
MYSPVQIANKFITLGNQHHNPLTHMQLQKLTYIAHGYYLALTGKPLLNECVSAWKYGPVIPGMYDAFKDYGNKPVTNVAV
APFGGIVTMDPQAESIIGAVYKFYGSKNGIELSTLTHMPGTPWSQAYNGIGSSIISNDAIKAYYHDLLNNRQQCQGL

Download         Length: 474 bp

>AT10007 NZ_AFEX01000045:54835-55308 [Escherichia coli STEC_O31]
ATGTACAGTCCGGTACAAATAGCCAATAAATTCATAACGTTGGGAAACCAACATCATAATCCTCTGACCCACATGCAACT
TCAGAAGCTTACTTATATTGCCCATGGTTACTATTTAGCATTAACAGGTAAGCCTTTGCTCAATGAATGTGTCTCCGCGT
GGAAGTACGGTCCAGTTATTCCTGGAATGTACGATGCTTTCAAAGACTATGGGAATAAACCTGTTACGAATGTAGCAGTA
GCTCCTTTTGGTGGCATCGTTACTATGGATCCGCAAGCAGAGAGCATTATAGGGGCTGTTTATAAGTTTTACGGCTCGAA
AAATGGAATTGAGTTATCAACTCTAACTCATATGCCAGGTACGCCTTGGTCACAAGCTTATAATGGTATTGGCTCGTCGA
TCATCTCAAATGATGCGATCAAGGCTTATTATCATGATTTATTGAATAACCGACAGCAATGTCAGGGCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT10009 Bartonella apis strain BBC0122

38.462

99.363

0.382
AT10013 Vibrio harveyi strain FDAARGOS_107

37.063

92.258

0.342
AT10010 Burkholderia prophage phi52237

31.818

98.089

0.312

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4KFL7

Antitoxin

Source ID Structure
AlphaFold DB A0A839B6W4

References

(1) Tatsuaki Kurata et al. (2022) A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains. Proceedings of The National Academy of Sciences of The United States of America 119(6):e2102212119. [PubMed:35121656] experimental


experimental experimental literature