Detailed information of TA system
Overview
TA module
| Type | VI | Classification (family/domain) | socAB/- |
| Location | 3785533..3786790 | Replicon | chromosome |
| Accession | NC_011916 | ||
| Organism | Caulobacter crescentus NA1000 | ||
Toxin (Protein)
| Gene name | socB | Uniprot ID | A0A0H3CC30 |
| Locus tag | CCNA_03629 | Protein ID | YP_002519002.1 |
| Coordinates | 3785533..3786246 (-) | Length | 238 a.a. |
Antitoxin (Protein)
| Gene name | socA | Uniprot ID | A0A0H3CCP8 |
| Locus tag | CCNA_03630 | Protein ID | YP_002519003.1 |
| Coordinates | 3786224..3786790 (-) | Length | 189 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CCNA_03626 | 3781650..3782612 | - | 963 | YP_002518999.1 | ATP phosphoribosyltransferase | - |
| CCNA_03627 | 3782612..3783772 | - | 1161 | YP_002519000.3 | ATP phosphoribosyltransferase regulatory subunit | - |
| CCNA_03628 | 3783744..3785231 | - | 1488 | YP_002519001.1 | histidyl-tRNA synthetase | - |
| CCNA_03629 | 3785533..3786246 | - | 714 | YP_002519002.1 | DNA replication inhibitor toxin SocB | Toxin |
| CCNA_03630 | 3786224..3786790 | - | 567 | YP_002519003.1 | antitoxin protein SocA | Antitoxin |
| CCNA_03631 | 3786985..3787089 | + | 105 | YP_002519004.1 | hypothetical protein | - |
| CCNA_03632 | 3787137..3787517 | + | 381 | YP_002519005.1 | hypothetical protein | - |
| CCNA_03633 | 3787860..3788231 | + | 372 | YP_002519006.1 | GIY-YIG domain protein | - |
| CCNA_03634 | 3788397..3788990 | - | 594 | YP_002519007.1 | DoxX family protein | - |
| CCNA_03996 | 3789044..3789103 | - | 60 | YP_009020568.1 | hypothetical protein | - |
| CCNA_03635 | 3789100..3789477 | - | 378 | YP_002519008.1 | hypothetical protein | - |
| CCNA_03636 | 3789689..3790714 | - | 1026 | YP_002519009.1 | biotin synthase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 238 a.a. Molecular weight: 27110.66 Da Isoelectric Point: 6.7466
>T6367 YP_002519002.1 NC_011916:c3786246-3785533 [Caulobacter vibrioides NA1000]
MKKHRLPDTELARIAPLAPDARRKALLKFKNGFPDFSYEPTRRRLPELTNAQPSLLSLGDTEWSKIESGLKRLKNEKEAA
SNIEVAELLYNFIREEKYIAVMEPFGKLQLGAGVAISYWSDAIFFGPDGPTIFGFDFRRAGGFNDSARRFAFSAQHEHIR
QRGDDYATAKLGLVQFPALRNGTRKVRVEFADQVELIPYDELIQMARETYSVWFEILEQREDEARKTGTGGSWWDGD
MKKHRLPDTELARIAPLAPDARRKALLKFKNGFPDFSYEPTRRRLPELTNAQPSLLSLGDTEWSKIESGLKRLKNEKEAA
SNIEVAELLYNFIREEKYIAVMEPFGKLQLGAGVAISYWSDAIFFGPDGPTIFGFDFRRAGGFNDSARRFAFSAQHEHIR
QRGDDYATAKLGLVQFPALRNGTRKVRVEFADQVELIPYDELIQMARETYSVWFEILEQREDEARKTGTGGSWWDGD
Download Length: 714 bp
>T6367 NC_011916:c3786246-3785533 [Caulobacter vibrioides NA1000]
TTGAAGAAGCACCGCCTTCCTGACACGGAACTGGCCCGTATTGCTCCCTTGGCGCCTGATGCTAGGCGCAAAGCGCTATT
GAAATTCAAGAATGGGTTTCCCGATTTCTCTTATGAGCCCACGCGACGTCGTCTGCCTGAGCTGACCAACGCCCAGCCCT
CCCTCCTGTCGCTTGGAGATACTGAGTGGAGTAAGATCGAGAGCGGCCTGAAAAGGCTCAAAAATGAGAAAGAGGCCGCC
TCAAATATAGAGGTCGCCGAGCTGCTGTATAATTTTATCAGAGAAGAAAAATACATCGCTGTCATGGAGCCATTTGGAAA
GCTCCAACTTGGTGCTGGCGTGGCGATATCATATTGGTCGGACGCGATTTTCTTTGGGCCAGATGGGCCAACGATATTCG
GCTTCGACTTTCGTCGGGCCGGAGGCTTTAACGATTCTGCTCGCCGCTTTGCTTTCTCTGCCCAGCACGAACATATTCGT
CAGAGAGGTGACGACTATGCCACCGCGAAACTCGGCTTGGTCCAATTTCCGGCGTTGCGAAATGGCACTAGAAAAGTGCG
TGTCGAGTTCGCGGATCAGGTCGAACTAATTCCGTACGATGAGCTGATCCAGATGGCCCGCGAGACGTACAGCGTTTGGT
TTGAAATCCTCGAACAGCGTGAGGATGAGGCCCGCAAGACGGGTACCGGTGGTTCCTGGTGGGATGGTGATTGA
TTGAAGAAGCACCGCCTTCCTGACACGGAACTGGCCCGTATTGCTCCCTTGGCGCCTGATGCTAGGCGCAAAGCGCTATT
GAAATTCAAGAATGGGTTTCCCGATTTCTCTTATGAGCCCACGCGACGTCGTCTGCCTGAGCTGACCAACGCCCAGCCCT
CCCTCCTGTCGCTTGGAGATACTGAGTGGAGTAAGATCGAGAGCGGCCTGAAAAGGCTCAAAAATGAGAAAGAGGCCGCC
TCAAATATAGAGGTCGCCGAGCTGCTGTATAATTTTATCAGAGAAGAAAAATACATCGCTGTCATGGAGCCATTTGGAAA
GCTCCAACTTGGTGCTGGCGTGGCGATATCATATTGGTCGGACGCGATTTTCTTTGGGCCAGATGGGCCAACGATATTCG
GCTTCGACTTTCGTCGGGCCGGAGGCTTTAACGATTCTGCTCGCCGCTTTGCTTTCTCTGCCCAGCACGAACATATTCGT
CAGAGAGGTGACGACTATGCCACCGCGAAACTCGGCTTGGTCCAATTTCCGGCGTTGCGAAATGGCACTAGAAAAGTGCG
TGTCGAGTTCGCGGATCAGGTCGAACTAATTCCGTACGATGAGCTGATCCAGATGGCCCGCGAGACGTACAGCGTTTGGT
TTGAAATCCTCGAACAGCGTGAGGATGAGGCCCGCAAGACGGGTACCGGTGGTTCCTGGTGGGATGGTGATTGA
Antitoxin
Download Length: 189 a.a. Molecular weight: 20423.76 Da Isoelectric Point: 9.5400
>AT6367 YP_002519003.1 NC_011916:c3786790-3786224 [Caulobacter vibrioides NA1000]
MPPLTQDPQSVDARAVANLLLDKAAALDIPISNLALQKLLYFAHGRFLVDKGRPLVNGFFEAWKFGPVHPVVYRCFSANG
PKYIINRAIKKDILSGLHIIVSPPRDQDIHEGIERVLLTMGRMSASQLVAVSHASGGPWDVIANGPGTNLGLGLRICDKV
IKDRFRFQKVSVSVPPGLGDTLEEAPPS
MPPLTQDPQSVDARAVANLLLDKAAALDIPISNLALQKLLYFAHGRFLVDKGRPLVNGFFEAWKFGPVHPVVYRCFSANG
PKYIINRAIKKDILSGLHIIVSPPRDQDIHEGIERVLLTMGRMSASQLVAVSHASGGPWDVIANGPGTNLGLGLRICDKV
IKDRFRFQKVSVSVPPGLGDTLEEAPPS
Download Length: 567 bp
>AT6367 NC_011916:c3786790-3786224 [Caulobacter vibrioides NA1000]
ATGCCTCCGCTGACTCAAGACCCTCAGTCGGTTGACGCGCGCGCAGTTGCCAACCTGCTCCTCGATAAGGCTGCGGCGCT
AGACATTCCGATAAGCAACCTAGCGCTCCAAAAGCTTCTTTACTTCGCGCACGGTCGCTTTCTTGTGGATAAAGGGCGCC
CCCTTGTAAACGGATTTTTCGAGGCTTGGAAATTCGGGCCAGTTCACCCTGTGGTTTATAGGTGCTTTAGTGCGAACGGA
CCTAAGTATATAATCAATAGAGCGATTAAGAAAGACATCCTGTCGGGCCTTCATATTATTGTATCTCCACCTCGTGACCA
GGATATTCATGAGGGCATCGAGCGTGTGCTGTTAACGATGGGGAGGATGTCTGCCTCTCAACTGGTCGCCGTCTCGCACG
CGTCTGGTGGTCCCTGGGACGTCATTGCGAACGGACCGGGAACAAATCTTGGGCTTGGGCTGCGGATATGCGATAAGGTG
ATCAAGGATCGATTCCGATTCCAGAAAGTCTCCGTAAGCGTTCCCCCCGGCCTTGGAGATACCCTTGAAGAAGCACCGCC
TTCCTGA
ATGCCTCCGCTGACTCAAGACCCTCAGTCGGTTGACGCGCGCGCAGTTGCCAACCTGCTCCTCGATAAGGCTGCGGCGCT
AGACATTCCGATAAGCAACCTAGCGCTCCAAAAGCTTCTTTACTTCGCGCACGGTCGCTTTCTTGTGGATAAAGGGCGCC
CCCTTGTAAACGGATTTTTCGAGGCTTGGAAATTCGGGCCAGTTCACCCTGTGGTTTATAGGTGCTTTAGTGCGAACGGA
CCTAAGTATATAATCAATAGAGCGATTAAGAAAGACATCCTGTCGGGCCTTCATATTATTGTATCTCCACCTCGTGACCA
GGATATTCATGAGGGCATCGAGCGTGTGCTGTTAACGATGGGGAGGATGTCTGCCTCTCAACTGGTCGCCGTCTCGCACG
CGTCTGGTGGTCCCTGGGACGTCATTGCGAACGGACCGGGAACAAATCTTGGGCTTGGGCTGCGGATATGCGATAAGGTG
ATCAAGGATCGATTCCGATTCCAGAAAGTCTCCGTAAGCGTTCCCCCCGGCCTTGGAGATACCCTTGAAGAAGCACCGCC
TTCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10010 | Burkholderia prophage phi52237 |
36.31 |
100 |
0.389 |
| AT10013 | Vibrio harveyi strain FDAARGOS_107 |
34.307 |
88.387 |
0.303 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3CC30 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3CCP8 |
References
(1) Christopher D Aakre et al. (2013) A bacterial toxin inhibits DNA replication elongation through a direct interaction with the β sliding clamp. Molecular Cell 52(5):617-28. [PubMed:24239291]