MGE detailed information
MGE type: Conjugative plasmid MGE length: 146245 bp
Accession: NZ_CP032080 Location: 1..146245 Organism: Escherichia coli strain CT30 Replicon: plasmid p.CT30-P2
Accession: NZ_CP032080 Location: 1..146245 Organism: Escherichia coli strain CT30 Replicon: plasmid p.CT30-P2
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D2195_RS24870 (246) | 246..1454 | + | 1209 | WP_021112574.1 | IS4 family transposase IS10R | Transposase |
| D2195_RS24875 (1967) | 1967..2166 | + | 200 | Protein_2 | lysis protein | - |
| D2195_RS24880 (2224) | 2224..2517 | - | 294 | WP_000738422.1 | increased serum survival lipoprotein Iss | - |
| D2195_RS24885 (3144) | 3144..4372 | + | 1229 | WP_212408153.1 | IS3 family transposase IS2 | Transposase |
| D2195_RS24890 (5596) | 5596..6789 | + | 1194 | WP_000450495.1 | GTP-binding protein | - |
| D2195_RS24895 (6967) | 6967..7116 | + | 150 | Protein_6 | IS3 family transposase IS2 | Transposase |
| D2195_RS24900 (7426) | 7426..8815 | + | 1390 | Protein_7 | ISNCY family transposase ISKpn21 | Transposase |
| D2195_RS24905 (9050) | 9050..9991 | + | 942 | WP_000974762.1 | hypothetical protein | - |
| D2195_RS24910 (10120) | 10120..10314 | - | 195 | Protein_9 | IS200-IS605 family transposase IS200C | Transposase |
| D2195_RS24915 (10788) | 10788..12158 | - | 1371 | WP_001312828.1 | efflux transporter outer membrane subunit | - |
| D2195_RS24920 (12162) | 12162..14102 | - | 1941 | WP_000733250.1 | Macrolide export ATP-binding/permease protein MacB | VF |
| D2195_RS24925 (14099) | 14099..15286 | - | 1188 | WP_000928803.1 | efflux RND transporter periplasmic adaptor subunit | - |
| D2195_RS26400 (15881) | 15881..16221 | - | 341 | Protein_13 | IS3 family transposase | - |
| D2195_RS24930 (16267) | 16267..16592 | + | 326 | Protein_14 | IS3 family transposase IS911 | Transposase |
| D2195_RS24940 (18222) | 18222..22355 | + | 4134 | WP_212408154.1 | Hemoglobin-binding protease hbp autotransporter | VF |
| D2195_RS24945 (22467) | 22467..23164 | + | 698 | WP_094096600.1 | IS1 family transposase IS1A | Transposase |
| D2195_RS26405 (23158) | 23158..23520 | - | 363 | Protein_18 | F-type conjugal transfer protein TrbF | - |
| D2195_RS24950 (23507) | 23507..23848 | - | 342 | WP_001442101.1 | P-type conjugative transfer protein TrbJ | - |
| D2195_RS24955 (23778) | 23778..24323 | - | 546 | WP_000059833.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| D2195_RS24960 (24310) | 24310..24594 | - | 285 | WP_000624108.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| D2195_RS24965 (24797) | 24797..25543 | - | 747 | WP_001016257.1 | IS21 family transposase ISEc12 | Transposase |
| D2195_RS24970 (25558) | 25558..27099 | - | 1542 | WP_001298859.1 | IS21 family transposase ISEc12 | Transposase |
| D2195_RS24975 (27345) | 27345..27620 | + | 276 | WP_001513501.1 | hypothetical protein | - |
| D2195_RS24980 (27622) | 27622..27969 | - | 348 | WP_001287907.1 | conjugal transfer protein TrbA | - |
| D2195_RS24985 (27985) | 27985..28728 | - | 744 | WP_001030367.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| D2195_RS24990 (28721) | 28721..28978 | - | 258 | WP_000864318.1 | conjugal transfer protein TrbE | - |
| D2195_RS24995 (29005) | 29005..30813 | - | 1809 | WP_000821847.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| D2195_RS25000 (30810) | 30810..31448 | - | 639 | WP_000777688.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| D2195_RS25005 (31457) | 31457..32449 | - | 993 | WP_000830187.1 | conjugal transfer pilus assembly protein TraU | - |
| D2195_RS25010 (32446) | 32446..33078 | - | 633 | WP_001203720.1 | type-F conjugative transfer system protein TraW | - |
| D2195_RS25015 (33075) | 33075..33461 | - | 387 | WP_000097319.1 | type-F conjugative transfer system protein TrbI | - |
| D2195_RS25020 (33458) | 33458..36085 | - | 2628 | WP_001064247.1 | type IV secretion system protein TraC | - |
| D2195_RS25025 (36245) | 36245..36466 | - | 222 | WP_001278689.1 | conjugal transfer protein TraR | - |
| D2195_RS25030 (36601) | 36601..37116 | - | 516 | WP_000809841.1 | type IV conjugative transfer system lipoprotein TraV | - |
| D2195_RS25035 (37113) | 37113..37364 | - | 252 | WP_001038342.1 | conjugal transfer protein TrbG | - |
| D2195_RS25040 (37376) | 37376..37573 | - | 198 | WP_001324648.1 | conjugal transfer protein TrbD | - |
| D2195_RS25045 (37560) | 37560..38150 | - | 591 | WP_000002792.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| D2195_RS25050 (38140) | 38140..39567 | - | 1428 | WP_000146692.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| D2195_RS25055 (39567) | 39567..40295 | - | 729 | WP_077520980.1 | type-F conjugative transfer system secretin TraK | - |
| D2195_RS25060 (40282) | 40282..40848 | - | 567 | WP_000399792.1 | type IV conjugative transfer system protein TraE | - |
| D2195_RS25065 (40870) | 40870..41181 | - | 312 | WP_000012106.1 | type IV conjugative transfer system protein TraL | - |
| D2195_RS25070 (41196) | 41196..41561 | - | 366 | WP_000994779.1 | type IV conjugative transfer system pilin TraA | - |
| D2195_RS25075 (41594) | 41594..41995 | - | 402 | WP_001369361.1 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
| D2195_RS25080 (42088) | 42088..42777 | - | 690 | WP_000283385.1 | conjugal transfer transcriptional regulator TraJ | - |
| D2195_RS25085 (42964) | 42964..43347 | - | 384 | WP_001151524.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| D2195_RS25090 (43668) | 43668..44270 | + | 603 | WP_000243709.1 | hypothetical protein | VF |
| D2195_RS25095 (44567) | 44567..45388 | - | 822 | WP_001234469.1 | DUF932 domain-containing protein | - |
| D2195_RS25100 (45510) | 45510..45797 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| D2195_RS25105 (45822) | 45822..46028 | - | 207 | WP_000547939.1 | hypothetical protein | - |
| D2195_RS26410 (46098) | 46098..46271 | + | 174 | Protein_51 | hypothetical protein | - |
| D2195_RS26415 (46269) | 46269..46499 | - | 231 | WP_001426396.1 | hypothetical protein | - |
| D2195_RS25110 (46719) | 46719..46844 | - | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| D2195_RS26420 (46786) | 46786..46935 | - | 150 | Protein_54 | plasmid maintenance protein Mok | - |
| - (46921) | 46921..47145 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (46921) | 46921..47145 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (46921) | 46921..47145 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (46921) | 46921..47145 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| D2195_RS25115 (46957) | 46957..47145 | + | 189 | WP_001299721.1 | hypothetical protein | - |
| D2195_RS25120 (47114) | 47114..47876 | - | 763 | Protein_56 | plasmid SOS inhibition protein A | - |
| D2195_RS25125 (47873) | 47873..48307 | - | 435 | WP_000845895.1 | conjugation system SOS inhibitor PsiB | - |
| D2195_RS25130 (48362) | 48362..50320 | - | 1959 | WP_001145469.1 | ParB/RepB/Spo0J family partition protein | - |
| D2195_RS25135 (50379) | 50379..50612 | - | 234 | WP_000006004.1 | DUF905 domain-containing protein | - |
| D2195_RS25140 (50668) | 50668..51195 | - | 528 | WP_000290793.1 | single-stranded DNA-binding protein | - |
| D2195_RS25145 (51668) | 51668..51928 | - | 261 | WP_072132736.1 | hypothetical protein | - |
| D2195_RS26425 (51838) | 51838..52083 | - | 246 | WP_241226192.1 | hypothetical protein | - |
| D2195_RS25150 (52080) | 52080..52643 | - | 564 | WP_001332335.1 | class I SAM-dependent methyltransferase | - |
| D2195_RS25155 (52691) | 52691..54052 | - | 1362 | WP_000170649.1 | DUF3560 domain-containing protein | - |
| D2195_RS25160 (54104) | 54104..54334 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| D2195_RS26430 (54851) | 54851..55104 | + | 254 | Protein_66 | hypothetical protein | - |
| D2195_RS25170 (55328) | 55328..55510 | - | 183 | WP_001027505.1 | hypothetical protein | - |
| D2195_RS25175 (55510) | 55510..55971 | - | 462 | WP_042007484.1 | DUF1380 family protein | - |
| D2195_RS25180 (55971) | 55971..56396 | - | 426 | WP_001198922.1 | antirestriction protein | - |
| D2195_RS25185 (56539) | 56539..57651 | + | 1113 | WP_021513474.1 | IS4 family transposase IS421 | Transposase |
| D2195_RS26435 (57844) | 57844..58013 | - | 170 | Protein_71 | hypothetical protein | - |
| D2195_RS25190 (58650) | 58650..59621 | - | 972 | WP_000817028.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| D2195_RS25195 (59621) | 59621..60787 | - | 1167 | WP_001238646.1 | Sporulation initiation inhibitor protein Soj | VF |
| D2195_RS25205 (61881) | 61881..62048 | - | 168 | WP_000638861.1 | hypothetical protein | - |
| D2195_RS25210 (62298) | 62298..63800 | - | 1503 | WP_000715078.1 | hypothetical protein | - |
| D2195_RS25215 (64417) | 64417..64842 | - | 426 | WP_012300440.1 | hypothetical protein | - |
| D2195_RS25220 (64984) | 64984..65463 | + | 480 | WP_000190054.1 | hypothetical protein | - |
| D2195_RS25225 (65903) | 65903..66565 | + | 663 | WP_000163251.1 | hypothetical protein | - |
| D2195_RS25235 (67798) | 67798..69999 | - | 2202 | WP_000973517.1 | Ferric aerobactin receptor | VF |
| D2195_RS25240 (70025) | 70025..71358 | - | 1334 | Protein_82 | L-lysine N6-monooxygenase | VF |
| D2195_RS25245 (71355) | 71355..73097 | - | 1743 | WP_001015721.1 | Aerobactin synthase | VF |
| D2195_RS25250 (73097) | 73097..74044 | - | 948 | WP_000011907.1 | N(6)-hydroxylysine O-acetyltransferase | VF |
| D2195_RS25255 (74045) | 74045..75769 | - | 1725 | WP_000602863.1 | N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase | VF |
| D2195_RS25260 (75905) | 75905..77098 | + | 1194 | WP_000095526.1 | MFS transporter | - |
| D2195_RS25265 (77478) | 77478..77858 | - | 381 | WP_001318207.1 | fluoride efflux transporter CrcB | - |
| D2195_RS25270 (78199) | 78199..78612 | - | 414 | Protein_88 | phosphopyruvate hydratase | - |
| D2195_RS25275 (78610) | 78610..78867 | + | 258 | Protein_89 | Tn3 family transposase | - |
| D2195_RS25280 (79101) | 79101..79958 | - | 858 | WP_000968139.1 | Manganese transport system membrane protein MntB | ARG |
| D2195_RS25285 (79955) | 79955..80812 | - | 858 | WP_001101723.1 | Manganese transport system membrane protein MntB | ARG |
| D2195_RS25290 (80809) | 80809..81636 | - | 828 | WP_000983712.1 | Iron(3+)-hydroxamate import ATP-binding protein FhuC | VF |
| D2195_RS25295 (81636) | 81636..82550 | - | 915 | WP_000949004.1 | Putative metal ABC transporter substrate-binding protein Hpf | VF |
| D2195_RS25300 (82906) | 82906..83603 | + | 698 | WP_223155668.1 | IS1 family transposase IS1A | Transposase |
| D2195_RS25305 (83783) | 83783..84296 | - | 514 | Protein_95 | DUF4113 domain-containing protein | - |
| D2195_RS25310 (84357) | 84357..84461 | - | 105 | Protein_96 | AAA family ATPase | - |
| D2195_RS25315 (84463) | 84463..84786 | - | 324 | Protein_97 | IS21 family transposase IS21 | Transposase |
| D2195_RS25320 (85532) | 85532..86509 | + | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| D2195_RS25325 (86794) | 86794..87534 | - | 741 | WP_001066954.1 | tyrosine-type recombinase/integrase | - |
| D2195_RS25330 (87655) | 87655..87843 | - | 189 | WP_001312821.1 | hypothetical protein | - |
| D2195_RS25335 (88217) | 88217..89119 | - | 903 | WP_001312822.1 | hypothetical protein | VF |
| D2195_RS25340 (89188) | 89188..90297 | - | 1110 | WP_000771475.1 | SDR family oxidoreductase | - |
| D2195_RS25345 (90730) | 90730..91683 | - | 954 | WP_000280980.1 | Protease 7 | VF |
| D2195_RS25350 (92980) | 92980..93159 | - | 180 | Protein_104 | integrase core domain-containing protein | - |
| D2195_RS25355 (93298) | 93298..94511 | + | 1214 | WP_162842477.1 | IS3 family transposase IS629 | Transposase |
| D2195_RS25360 (94780) | 94780..95067 | - | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| D2195_RS25365 (95064) | 95064..95315 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| D2195_RS25370 (96278) | 96278..97135 | - | 858 | WP_000774297.1 | incFII family plasmid replication initiator RepA | - |
| D2195_RS25375 (97128) | 97128..97610 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| D2195_RS26440 (97603) | 97603..97650 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| D2195_RS26445 (97641) | 97641..97892 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| D2195_RS25385 (97909) | 97909..98166 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| D2195_RS25390 (98450) | 98450..98599 | - | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin Detail |
| - (98643) | 98643..98704 | + | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (98643) | 98643..98704 | + | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (98643) | 98643..98704 | + | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (98643) | 98643..98704 | + | 62 | NuclAT_1 | - | Antitoxin Detail |
| D2195_RS25395 (98960) | 98960..99034 | - | 75 | Protein_114 | endonuclease | - |
| D2195_RS25400 (99280) | 99280..99492 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| D2195_RS25405 (99628) | 99628..100188 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
| D2195_RS25410 (100291) | 100291..101151 | - | 861 | WP_000704513.1 | putative protein YcjY | VF |
| D2195_RS25415 (101210) | 101210..101956 | - | 747 | WP_000205749.1 | conjugal transfer pilus acetylase TraX | - |
| D2195_RS25420 (101976) | 101976..107246 | - | 5271 | WP_021513980.1 | Multifunctional conjugation protein TraI | Relaxase |
| D2195_RS25425 (107328) | 107328..107555 | + | 228 | WP_000450520.1 | toxin-antitoxin system antitoxin VapB | - |
| D2195_RS25430 (107555) | 107555..107953 | + | 399 | WP_000911333.1 | tRNA(fMet)-specific endonuclease VapC | Toxin Detail |
| D2195_RS25435 (107962) | 107962..110115 | - | 2154 | WP_000009325.1 | type IV conjugative transfer system coupling protein TraD | - |
| D2195_RS25440 (110368) | 110368..111099 | - | 732 | WP_000850422.1 | conjugal transfer complement resistance protein TraT | - |
| D2195_RS25445 (111113) | 111113..111622 | - | 510 | WP_021513982.1 | conjugal transfer entry exclusion protein TraS | - |
| D2195_RS25450 (111619) | 111619..114444 | - | 2826 | WP_212408152.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| D2195_RS25455 (114441) | 114441..115814 | - | 1374 | WP_000944319.1 | conjugal transfer pilus assembly protein TraH | - |
| D2195_RS25460 (115917) | 115917..116614 | + | 698 | WP_094096600.1 | IS1 family transposase IS1A | Transposase |
| D2195_RS25465 (116639) | 116639..117661 | - | 1023 | WP_000361402.1 | helicase UvrD | - |
| D2195_RS25470 (117646) | 117646..119211 | - | 1566 | WP_001128474.1 | AAA family ATPase | - |
| D2195_RS25475 (119286) | 119286..119702 | - | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | Toxin Detail |
| D2195_RS25480 (119699) | 119699..119929 | - | 231 | WP_001261286.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| D2195_RS25485 (120098) | 120098..120424 | + | 327 | WP_249534284.1 | hypothetical protein | - |
| D2195_RS25490 (120421) | 120421..121212 | + | 792 | WP_000016494.1 | site-specific integrase | - |
| D2195_RS25495 (121390) | 121390..121743 | + | 354 | WP_000864812.1 | colicin M immunity protein | - |
| D2195_RS25500 (121793) | 121793..122608 | - | 816 | WP_001312845.1 | lipid II-degrading bacteriocin colicin M | - |
| D2195_RS25505 (122852) | 122852..123379 | + | 528 | WP_000203272.1 | colicin B immunity protein | - |
| D2195_RS25510 (123397) | 123397..123564 | - | 168 | Protein_137 | colicin-B | - |
| D2195_RS25515 (123737) | 123737..123940 | + | 204 | Protein_138 | hypothetical protein | - |
| D2195_RS25520 (124564) | 124564..125550 | - | 987 | Protein_139 | IS4 family transposase IS421 | Transposase |
| D2195_RS26450 (125731) | 125731..126003 | - | 273 | Protein_140 | IS4 family transposase IS421 | Transposase |
| D2195_RS25530 (126110) | 126110..127384 | - | 1275 | WP_000489607.1 | HlyD family secretion protein | - |
| D2195_RS25540 (129331) | 129331..129600 | + | 270 | WP_000079941.1 | type II toxin-antitoxin system antitoxin YacA | - |
| D2195_RS25545 (129600) | 129600..129878 | + | 279 | WP_097309154.1 | type II toxin-antitoxin system toxin YacB | Toxin Detail |
| D2195_RS25550 (129924) | 129924..130772 | + | 849 | WP_001058005.1 | 3'-5' exonuclease | - |
| D2195_RS25555 (130889) | 130889..131371 | + | 483 | WP_001311056.1 | hypothetical protein | - |
| D2195_RS25560 (131673) | 131673..132020 | + | 348 | WP_000142437.1 | DUF6404 family protein | - |
| D2195_RS26455 (132098) | 132098..132398 | - | 301 | Protein_148 | hypothetical protein | - |
| D2195_RS25565 (132579) | 132579..133520 | + | 942 | WP_001312841.1 | 3-deoxy-7-phosphoheptulonate synthase | - |
| D2195_RS26460 (133480) | 133480..133614 | + | 135 | WP_249531455.1 | hypothetical protein | - |
| D2195_RS25570 (134469) | 134469..136646 | + | 2178 | WP_001222186.1 | Ferric enterobactin receptor | VF |
| D2195_RS25575 (136691) | 136691..137647 | - | 957 | WP_000271276.1 | Ferri-bacillibactin esterase BesA | VF |
| D2195_RS25580 (137732) | 137732..138961 | - | 1230 | WP_000933675.1 | Enterochelin esterase | VF |
| D2195_RS25585 (139065) | 139065..142724 | - | 3660 | WP_001111199.1 | Multidrug efflux ATP-binding/permease protein | VF |
| D2195_RS25590 (142864) | 142864..143979 | - | 1116 | WP_001318220.1 | Elloramycin glycosyltransferase ElmGT | VF |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|