Detailed information of TA system TA111046

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46719..47145
• Replicon: plasmid p.CT30-P2
• Accession: NZ_CP032080
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: D2195_RS25110
• Protein ID: WP_001372321.1
• Coordinates: 46719..46844 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	46921..47145 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS25080 (42088)	42088..42777	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
D2195_RS25085 (42964)	42964..43347	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
D2195_RS25090 (43668)	43668..44270	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
D2195_RS25095 (44567)	44567..45388	-	822	WP_001234469.1	DUF932 domain-containing protein	-
D2195_RS25100 (45510)	45510..45797	-	288	WP_000107535.1	hypothetical protein	-
D2195_RS25105 (45822)	45822..46028	-	207	WP_000547939.1	hypothetical protein	-
D2195_RS26410 (46098)	46098..46271	+	174	Protein_51	hypothetical protein	-
D2195_RS26415 (46269)	46269..46499	-	231	WP_001426396.1	hypothetical protein	-
D2195_RS25110 (46719)	46719..46844	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
D2195_RS26420 (46786)	46786..46935	-	150	Protein_54	plasmid maintenance protein Mok	-
- (46921)	46921..47145	-	225	NuclAT_0	-	Antitoxin
- (46921)	46921..47145	-	225	NuclAT_0	-	Antitoxin
- (46921)	46921..47145	-	225	NuclAT_0	-	Antitoxin
- (46921)	46921..47145	-	225	NuclAT_0	-	Antitoxin
D2195_RS25115 (46957)	46957..47145	+	189	WP_001299721.1	hypothetical protein	-
D2195_RS25120 (47114)	47114..47876	-	763	Protein_56	plasmid SOS inhibition protein A	-
D2195_RS25125 (47873)	47873..48307	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
D2195_RS25130 (48362)	48362..50320	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
D2195_RS25135 (50379)	50379..50612	-	234	WP_000006004.1	DUF905 domain-containing protein	-
D2195_RS25140 (50668)	50668..51195	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
D2195_RS25145 (51668)	51668..51928	-	261	WP_072132736.1	hypothetical protein	-
D2195_RS26425 (51838)	51838..52083	-	246	WP_241226192.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iutA / iucD / iucC / iucB / iucA / iroN / iroE / iroD / iroC / iroB	1..146245	146245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T111046 WP_001372321.1 NZ_CP032080:c46844-46719 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T111046 NZ_CP032080:c46844-46719 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT111046 NZ_CP032080:c47145-46921 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin