Detailed information of TA system TA111056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 129331..129878
• Replicon: plasmid p.CT30-P2
• Accession: NZ_CP032080
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: D2195_RS25545
• Protein ID: WP_097309154.1
• Coordinates: 129600..129878 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: D2195_RS25540
• Protein ID: WP_000079941.1
• Coordinates: 129331..129600 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS25520 (124564)	124564..125550	-	987	Protein_139	IS4-like element IS421 family transposase	-
D2195_RS26450 (125731)	125731..126003	-	273	Protein_140	cysteine peptidase family C39 domain-containing protein	-
D2195_RS25530 (126110)	126110..127384	-	1275	WP_000489607.1	HlyD family secretion protein	-
D2195_RS25540 (129331)	129331..129600	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
D2195_RS25545 (129600)	129600..129878	+	279	WP_097309154.1	type II toxin-antitoxin system toxin YacB	Toxin
D2195_RS25550 (129924)	129924..130772	+	849	WP_001058005.1	3'-5' exonuclease	-
D2195_RS25555 (130889)	130889..131371	+	483	WP_001311056.1	hypothetical protein	-
D2195_RS25560 (131673)	131673..132020	+	348	WP_000142437.1	DUF6404 family protein	-
D2195_RS26455 (132098)	132098..132398	-	301	Protein_148	hypothetical protein	-
D2195_RS25565 (132579)	132579..133520	+	942	WP_001312841.1	3-deoxy-7-phosphoheptulonate synthase	-
D2195_RS26460 (133480)	133480..133614	+	135	WP_249531455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iutA / iucD / iucC / iucB / iucA / iroN / iroE / iroD / iroC / iroB	1..146245	146245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10799.42 Da
• Isoelectric Point: 8.4615

>T111056 WP_097309154.1 NZ_CP032080:129600-129878 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T111056 NZ_CP032080:129600-129878 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT111056 WP_000079941.1 NZ_CP032080:129331-129600 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT111056 NZ_CP032080:129331-129600 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5