Detailed information of TA system TA111055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119286..119929
• Replicon: plasmid p.CT30-P2
• Accession: NZ_CP032080
• Organism: Escherichia coli strain CT30

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: D2195_RS25475
• Protein ID: WP_001034044.1
• Coordinates: 119286..119702 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: D2195_RS25480
• Protein ID: WP_001261286.1
• Coordinates: 119699..119929 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2195_RS25455 (114441)	114441..115814	-	1374	WP_000944319.1	conjugal transfer pilus assembly protein TraH	-
D2195_RS25460 (115917)	115917..116614	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
D2195_RS25465 (116639)	116639..117661	-	1023	WP_000361402.1	helicase UvrD	-
D2195_RS25470 (117646)	117646..119211	-	1566	WP_001128474.1	AAA family ATPase	-
D2195_RS25475 (119286)	119286..119702	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
D2195_RS25480 (119699)	119699..119929	-	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
D2195_RS25485 (120098)	120098..120424	+	327	WP_249534284.1	hypothetical protein	-
D2195_RS25490 (120421)	120421..121212	+	792	WP_000016494.1	site-specific integrase	-
D2195_RS25495 (121390)	121390..121743	+	354	WP_000864812.1	colicin M immunity protein	-
D2195_RS25500 (121793)	121793..122608	-	816	WP_001312845.1	lipid II-degrading bacteriocin colicin M	-
D2195_RS25505 (122852)	122852..123379	+	528	WP_000203272.1	colicin B immunity protein	-
D2195_RS25510 (123397)	123397..123564	-	168	Protein_137	colicin-B	-
D2195_RS25515 (123737)	123737..123940	+	204	Protein_138	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iutA / iucD / iucC / iucB / iucA / iroN / iroE / iroD / iroC / iroB	1..146245	146245
	-	flank	IS/Tn	-	-	116237..116614	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T111055 WP_001034044.1 NZ_CP032080:c119702-119286 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T111055 NZ_CP032080:c119702-119286 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT111055 WP_001261286.1 NZ_CP032080:c119929-119699 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT111055 NZ_CP032080:c119929-119699 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6