Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 94780..95315 Replicon plasmid p.CT30-P2
Accession NZ_CP032080
Organism Escherichia coli strain CT30

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag D2195_RS25360 Protein ID WP_000222766.1
Coordinates 94780..95067 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag D2195_RS25365 Protein ID WP_001132900.1
Coordinates 95064..95315 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2195_RS25345 (90730) 90730..91683 - 954 WP_000280980.1 omptin family outer membrane protease -
D2195_RS25350 (92980) 92980..93159 - 180 Protein_104 integrase core domain-containing protein -
D2195_RS25355 (93298) 93298..94511 + 1214 WP_162842477.1 IS3 family transposase -
D2195_RS25360 (94780) 94780..95067 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D2195_RS25365 (95064) 95064..95315 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D2195_RS25370 (96278) 96278..97135 - 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
D2195_RS25375 (97128) 97128..97610 - 483 WP_001273588.1 hypothetical protein -
D2195_RS26440 (97603) 97603..97650 - 48 WP_229471593.1 hypothetical protein -
D2195_RS26445 (97641) 97641..97892 + 252 WP_223195197.1 replication protein RepA -
D2195_RS25385 (97909) 97909..98166 - 258 WP_000083845.1 replication regulatory protein RepA -
D2195_RS25390 (98450) 98450..98599 - 150 WP_001312851.1 Hok/Gef family protein -
- (98643) 98643..98704 + 62 NuclAT_1 - -
- (98643) 98643..98704 + 62 NuclAT_1 - -
- (98643) 98643..98704 + 62 NuclAT_1 - -
- (98643) 98643..98704 + 62 NuclAT_1 - -
D2195_RS25395 (98960) 98960..99034 - 75 Protein_114 endonuclease -
D2195_RS25400 (99280) 99280..99492 - 213 WP_001299730.1 ANR family transcriptional regulator -
D2195_RS25405 (99628) 99628..100188 - 561 WP_000139315.1 fertility inhibition protein FinO -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iutA / iucD / iucC / iucB / iucA / iroN / iroE / iroD / iroC / iroB 1..146245 146245


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-83)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T111049 WP_000222766.1 NZ_CP032080:c95067-94780 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T111049 NZ_CP032080:c95067-94780 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT111049 WP_001132900.1 NZ_CP032080:c95315-95064 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT111049 NZ_CP032080:c95315-95064 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2


Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References