SecReT4
T4SS ID378
StrainBrucella suis 1330
Repliconchromosome II [Browse all T4SS(s) in this replicon]
AccessionNC_004311
Location56802..67241
NameVirB
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.
structure Information of structure: structureVirB11, VirB8

T4SS components
ComponentVirB1VirB2VirB3VirB4VirB5VirB6VirB7VirB8VirB9
Number111111111
ComponentVirB10VirB11VirD4
Number110

The information of T4SS components from NC_004311
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1BRA005653108..54511 [-], 140423499823amino acid permease 
2BRA005754733..55884 [-], 115223499824hypothetical protein 
3BRA005856135..56653 [-], 51923499825lipoprotein 
4BRA005956802..57887 [-], 108623499826type IV secretion system protein VirB11  VirB11
5BRA006057868..59043 [-], 117623499827type IV secretion system protein VirB10  VirB10
6BRA006159040..59909 [-], 87023499828type IV secretion system protein VirB9  VirB9
7BRA006259906..60625 [-], 72023499829type IV secretion system protein VirB8  VirB8
8BRA006360628..60801 [-], 17423499830type IV secretion system protein VirB7  VirB7
9BRA006460965..62008 [-], 104423499831type IV secretion system protein VirB6  VirB6
10BRA006562192..62908 [-], 71723499832type IV secretion system protein VirB5  VirB5
11BRA006662913..65408 [-], 249623499833type IV secretion system protein VirB4  VirB4
12BRA006765408..65758 [-], 35123499834type IV secretion system protein VirB3  VirB3
13BRA006865772..66089 [-], 31823499835type IV secretion system protein VirB2  VirB2
14BRA006966525..67241 [-], 71723499836type IV secretion system protein VirB1  VirB1
15BRA007067745..68971 [-], 122723499837transglycosylase 
16BRA0071 (galU)69383..70276 [+], 89423499838UTP-glucose-1-phosphate uridylyltransferase 
17BRA007270329..71867 [-], 153923499839hypothetical protein 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
BPE123, BtpB, BPE043, VceC, BPE275, RicA, VceA, BPE005

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1BRA0124 122260..122721 [-], 46223499887hypothetical protein  BPE123
2BR0735 721187..722020 [+], 83423501622hypothetical proteinexperimental  BtpB
3BR1024 988677..993350 [-], 467423501904hypothetical protein  BPE043
4BR1038 1002486..1003718 [-], 123323501917hypothetical protein  VceC
5BR1256 1222086..1222847 [-], 76223502132rhomboid family protein  BPE275
6BR1260 1224775..1225302 [-], 52823502136ferripyochelin-binding protein  RicA
7BR1634 1581792..1582109 [+], 31823502495hypothetical protein  VceA
8BR2005 1927799..1928260 [-], 46223502853cyclic nucleotide-binding protein  BPE005


Download FASTA format files
Proteins        Genes
The information on structure of this T4ss

#NameImageResourceDetailReference
1VirB112GZA PDB (2GZA)Crystal structure of the VirB11 ATPase from the Brucella Suis type IV secretion system in complex with sulphate(1) PubMed: 16730027
2VirB82BHM PDB (2BHM)Crystal structure of VirB8 (2) PubMed: 15764702
(1) Hare S; Bayliss R; Baron C; Waksman G (2006). A large domain swap in the VirB11 ATPase of Brucella suis leaves the hexameric assembly intact. J Mol Biol. 360(1):56-66. [PudMed:16730027] experimental
(2) Terradot L; Bayliss R; Oomen C; Leonard GA; Baron C; Waksman G (2005). Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori. Proc Natl Acad Sci U S A. 102(12):4596-601. [PudMed:15764702] experimental
(1) Chandran Darbari V et al. (2015). Structural Biology of Bacterial Type IV Secretion Systems. Annu Rev Biochem. 84:603-29. [PudMed:26034891]
(2) Villamil Giraldo AM; Sivanesan D; Carle A; Paschos A; Smith MA; Plesa M; Coulton J; Baron C (2012). Type IV Secretion System Core Component VirB8 from Brucella Binds to the Globular Domain of VirB5 and to a Periplasmic Domain of VirB6. Biochemistry. 51(18):3881-90. [PudMed:22515661] experimental
(3) de Jong MF; Tsolis RM (2012). Brucellosis and type IV secretion. Future Microbiol. 7(1):47-58. [PudMed:22191446]
(4) Andrieux L; Bourg G; Pirone A; O'Callaghan D; Patey G (2011). A single amino acid change in the transmembrane domain of the VirB8 protein affects dimerization, interaction with VirB10 and Brucella suis virulence. FEBS Lett. 585(15):2431-6. [PudMed:21763312] experimental
(5) Sivanesan D; Hancock MA; Villamil Giraldo AM; Baron C (2010). Quantitative analysis of VirB8-VirB9-VirB10 interactions provides a dynamic model of type IV secretion system core complex assembly. Biochemistry. 49(21):4483-93. [PudMed:20426418] experimental
(6) Bourg G; Sube R; O'Callaghan D; Patey G (2009). Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems. J Bacteriol. 191(9):2985-92. [PudMed:19251859] experimental
(7) Dozot M; Boigegrain RA; Delrue RM; Hallez R; Ouahrani-Bettache S; Danese I; Letesson JJ; De Bolle X; Kohler S (2006). The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB. Cell Microbiol. 8(11):1791-802. [PudMed:16803581] experimental
(8) Hare S; Bayliss R; Baron C; Waksman G (2006). A large domain swap in the VirB11 ATPase of Brucella suis leaves the hexameric assembly intact. J Mol Biol. 360(1):56-66. [PudMed:16730027] experimental
(9) Paschos A; Patey G; Sivanesan D; Gao C; Bayliss R; Waksman G; O'callaghan D; Baron C (2006). Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proc Natl Acad Sci U S A. 103(19):7252-7. [PudMed:16648257] experimental
(10) Carle A; Hoppner C; Ahmed Aly K; Yuan Q; den Dulk-Ras A; Vergunst A; O'Callaghan D; Baron C (2006). The Brucella suis type IV secretion system assembles in the cell envelope of the heterologous host Agrobacterium tumefaciens and increases IncQ plasmid pLS1 recipient competence. Infect Immun. 74(1):108-17. [PudMed:16368963] experimental
(11) de Paz HD; Sangari FJ; Bolland S; Garcia-Lobo JM; Dehio C; de la Cruz F; Llosa M (2005). Functional interactions between type IV secretion systems involved in DNA transfer and virulence. Microbiology. 151(Pt 11):3505-16. [PudMed:16272374] experimental
(12) Hoppner C; Carle A; Sivanesan D; Hoeppner S; Baron C (2005). The putative lytic transglycosylase VirB1 from Brucella suis interacts with the type IV secretion system core components VirB8, VirB9 and VirB11. Microbiology. 151(Pt 11):3469-82. [PudMed:16272371] experimental
(13) Zahrl D; Wagner M; Bischof K; Bayer M; Zavecz B; Beranek A; Ruckenstuhl C; Zarfel GE; Koraimann G (2005). Peptidoglycan degradation by specialized lytic transglycosylases associated with type III and type IV secretion systems. Microbiology. 151(Pt 11):3455-67. [PudMed:16272370] experimental
(14) Sun YH; Rolan HG; den Hartigh AB; Sondervan D; Tsolis RM (2005). Brucella abortus virB12 is expressed during infection but is not an essential component of the type IV secretion system. Infect Immun. 73(9):6048-54. [PudMed:16113325] experimental
(15) Terradot L; Bayliss R; Oomen C; Leonard GA; Baron C; Waksman G (2005). Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori. Proc Natl Acad Sci U S A. 102(12):4596-601. [PudMed:15764702] experimental
(16) Rouot B; Alvarez-Martinez MT; Marius C; Menanteau P; Guilloteau L; Boigegrain RA; Zumbihl R; O'Callaghan D; Domke N; Baron C (2003). Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera. Infect Immun. 71(3):1075-82. [PudMed:12595417] experimental
(17) Boschiroli ML; Ouahrani-Bettache S; Foulongne V; Michaux-Charachon S; Bourg G; Allardet-Servent A; Cazevieille C; Liautard JP; Ramuz M; O'Callaghan D (2002). The Brucella suis virB operon is induced intracellularly in macrophages. Proc Natl Acad Sci U S A. 99(3):1544-9. [PudMed:11830669] experimental
(18) O'Callaghan D; Cazevieille C; Allardet-Servent A; Boschiroli ML; Bourg G; Foulongne V; Frutos P; Kulakov Y; Ramuz M (1999). A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Mol Microbiol. 33(6):1210-20. [PudMed:10510235] experimental
 
experimental This literature contains experimental investigation

Genomics & Bioinformatics Group in MML, SJTU