StrainAgrobacterium tumefaciens plasmid Ti plasmid pTiBo542 (NC_010929)
Repliconplasmid Ti plasmid pTiBo542 [Browse all T4SS(s) in this replicon]
Functioneffector translocation and conjugation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_010929
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pTiBo128148219..148605 [+], 387190014762orf_Bo128 
2pTiBo129148602..148946 [+], 345190014763orf_Bo129 
3pTiBo130149021..150610 [+], 1590190014764orf_Bo130 
4pTiBo131 (virJ)150873..151616 [+], 744190014765VirJ 
5pTiBo132151620..151997 [-], 378190014766orf_Bo132 
6pTiBo133 (virB1)153093..153812 [+], 720190014767type IV secretion system lytic transglycosylase VirB1  VirB1
7pTiBo134 (virB2)153827..154192 [+], 366190014768type IV secretion system pilin subunit VirB2  VirB2
8pTiBo135 (virB3)154192..154518 [+], 327190014769type IV secretion system protein VirB3  VirB3
9pTiBo136 (virB4)154518..156887 [+], 2370190014770type IV secretion system protein VirB4  VirB4
10pTiBo137 (virB5)156902..157564 [+], 663190014771type IV secretion system protein VirB5  VirB5
11pTiBo138 (virB6)157661..158548 [+], 888190014772type IV secretion system protein VirB6  VirB6
12pTiBo139 (virB7)158582..158749 [+], 168190014773type IV secretion system lipoprotein VirB7  VirB7
13pTiBo140 (virB8)158736..159449 [+], 714190014774type IV secretion system protein VirB8  VirB8
14pTiBo141 (virB9)159446..160327 [+], 882190014775type IV secretion system protein VirB9  VirB9
15pTiBo142 (virB10)160324..161457 [+], 1134190014776type IV secretion system protein VirB10  VirB10
16pTiBo143 (virB11)161497..162528 [+], 1032190014777type IV secretion system protein VirB11  VirB11
17pTiBo144 (virG)162660..163463 [+], 804190014778two-component response regulator VirG 
18pTiBo145 (virC2)163574..164182 [-], 609190014779putative crown gall tumor protein VirC2 
19pTiBo146 (virC1)164185..164880 [-], 696190014780putative crown gall tumor protein VirC1  accesspry protein
20pTiBo147 (virD1)165149..165592 [+], 444190014781type IV secretion system T-DNA border endonuclease VirD1 
21pTiBo148 (virD2)165626..166900 [+], 1275190014782type IV secretion system T-DNA border endonuclease VirD2 
22pTiBo149 (virD3)166920..167558 [+], 639190014783VirD3 
23pTiBo150 (virD4)167555..169513 [+], 1959190014784type IV secretion system protein VirD4  VirD4
24pTiBo151 (virD5)169614..172124 [+], 2511190014785VirD5 
25pTiBo152 (virE1)172574..172771 [+], 198190014786type IV secretion system chaperone VirE1  accesspry protein
26pTiBo153 (virE2)172776..174425 [+], 1650190014787type IV secretion system single-stranded DNA binding protein VirE2 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
This system delivers both DNA and proteins into plant cell. The transferred DNA (T-DNA) region: NC_010929: 1..15730)

VirD2, VirD5, VirE2, VirE3, VirF

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1pTiBo148 (virD2)165626..166900 [+], 1275190014782type IV secretion system T-DNA border endonuclease VirD2  VirD2
2pTiBo151 (virD5)169614..172124 [+], 2511190014785VirD5  VirD5
3pTiBo153 (virE2)172776..174425 [+], 1650190014787type IV secretion system single-stranded DNA binding protein VirE2  VirE2
4pTiBo154 (virE3)174492..176510 [+], 2019190014788VirE3  VirE3
5pTiBo210 222375..223316 [+], 942190014842orf_Bo210  VirF

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1VirC1 (190014780)NDVirD2VirC1 pairwise interacts with the processing factors VirD2 relaxase, VirC2, and VirD1 to stimulate generation of multiple copies per cell of the T-DNA substrate. VirC1 promotes conjugative T-DNA transfer.(1) PubMed: 17505518
2VirE1 chaperone (190014786)CT50aa sufficient (interacts with the VirD4 T4CP. CT100aa(VirE2) sufficient for the T4CP interation)VirE2Translocation of the VirE2 effector through the A. tumefaciens VirB/VirD4 T4SS requires VirE1.(2) PubMed: 19946141
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Atmakuri K; Cascales E; Burton OT; Banta LM; Christie PJ (2007). Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. EMBO J. 26(10):2540-51. [PudMed:17505518] experimental
(2) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]
(1) Mossey P; Hudacek A; Das A (2010). Agrobacterium tumefaciens type IV secretion protein VirB3 is an inner membrane protein and requires VirB4, VirB7, and VirB8 for stabilization. J Bacteriol. 192(11):2830-8. [PudMed:20348257] experimental
experimental This literature contains experimental investigation