T4SS ID	15
Strain	Agrobacterium tumefaciens plasmid Ti plasmid pTiBo542 (NC_010929)
Replicon	plasmid Ti plasmid pTiBo542 [Browse all T4SS(s) in this replicon]
Accession	NC_010929
Location	153093..169513
Name	VirB
Function	effector translocation and conjugation
Classification	Type IVA; Type P
Experimental investigation has been performed on this T4SS.

T4SS components

Component	VirB1	VirB2	VirB3	VirB4	VirB5	VirB6	VirB7	VirB8	VirB9
Number	1	1	1	1	1	1	1	1	1
Component	VirB10	VirB11	VirD4
Number	1	1	1

The information of T4SS components from NC_010929
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pTiBo128	148219..148605 [+], 387	190014762	orf_Bo128
2	pTiBo129	148602..148946 [+], 345	190014763	orf_Bo129
3	pTiBo130	149021..150610 [+], 1590	190014764	orf_Bo130
4	pTiBo131 (virJ)	150873..151616 [+], 744	190014765	VirJ
5	pTiBo132	151620..151997 [-], 378	190014766	orf_Bo132
6	pTiBo133 (virB1)	153093..153812 [+], 720	190014767	type IV secretion system lytic transglycosylase VirB1	VirB1
7	pTiBo134 (virB2)	153827..154192 [+], 366	190014768	type IV secretion system pilin subunit VirB2	VirB2
8	pTiBo135 (virB3)	154192..154518 [+], 327	190014769	type IV secretion system protein VirB3	VirB3
9	pTiBo136 (virB4)	154518..156887 [+], 2370	190014770	type IV secretion system protein VirB4	VirB4
10	pTiBo137 (virB5)	156902..157564 [+], 663	190014771	type IV secretion system protein VirB5	VirB5
11	pTiBo138 (virB6)	157661..158548 [+], 888	190014772	type IV secretion system protein VirB6	VirB6
12	pTiBo139 (virB7)	158582..158749 [+], 168	190014773	type IV secretion system lipoprotein VirB7	VirB7
13	pTiBo140 (virB8)	158736..159449 [+], 714	190014774	type IV secretion system protein VirB8	VirB8
14	pTiBo141 (virB9)	159446..160327 [+], 882	190014775	type IV secretion system protein VirB9	VirB9
15	pTiBo142 (virB10)	160324..161457 [+], 1134	190014776	type IV secretion system protein VirB10	VirB10
16	pTiBo143 (virB11)	161497..162528 [+], 1032	190014777	type IV secretion system protein VirB11	VirB11
17	pTiBo144 (virG)	162660..163463 [+], 804	190014778	two-component response regulator VirG
18	pTiBo145 (virC2)	163574..164182 [-], 609	190014779	putative crown gall tumor protein VirC2
19	pTiBo146 (virC1)	164185..164880 [-], 696	190014780	putative crown gall tumor protein VirC1
20	pTiBo147 (virD1)	165149..165592 [+], 444	190014781	type IV secretion system T-DNA border endonuclease VirD1
21	pTiBo148 (virD2)	165626..166900 [+], 1275	190014782	type IV secretion system T-DNA border endonuclease VirD2
22	pTiBo149 (virD3)	166920..167558 [+], 639	190014783	VirD3
23	pTiBo150 (virD4)	167555..169513 [+], 1959	190014784	type IV secretion system protein VirD4	VirD4
24	pTiBo151 (virD5)	169614..172124 [+], 2511	190014785	VirD5
25	pTiBo152 (virE1)	172574..172771 [+], 198	190014786	type IV secretion system chaperone VirE1
26	pTiBo153 (virE2)	172776..174425 [+], 1650	190014787	type IV secretion system single-stranded DNA binding protein VirE2

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

This system delivers both DNA and proteins into plant cell. The transferred DNA (T-DNA) region: NC_010929: 1..15730)

Effectors

VirD2, VirD5, VirE2, VirE3, VirF

The information of protein effectors
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	*
1	pTiBo148 (virD2)	165626..166900 [+], 1275	190014782	type IV secretion system T-DNA border endonuclease VirD2	VirD2
2	pTiBo151 (virD5)	169614..172124 [+], 2511	190014785	VirD5	VirD5
3	pTiBo153 (virE2)	172776..174425 [+], 1650	190014787	type IV secretion system single-stranded DNA binding protein VirE2	VirE2
4	pTiBo154 (virE3)	174492..176510 [+], 2019	190014788	VirE3	VirE3
5	pTiBo210	222375..223316 [+], 942	190014842	orf_Bo210	VirF

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	VirC1 (190014780)	ND	VirD2	VirC1 pairwise interacts with the processing factors VirD2 relaxase, VirC2, and VirD1 to stimulate generation of multiple copies per cell of the T-DNA substrate. VirC1 promotes conjugative T-DNA transfer.	(1) PubMed: 17505518
2	VirE1 chaperone (190014786)	CT50aa sufficient (interacts with the VirD4 T4CP. CT100aa(VirE2) sufficient for the T4CP interation)	VirE2	Translocation of the VirE2 effector through the A. tumefaciens VirB/VirD4 T4SS requires VirE1.	(2) PubMed: 19946141

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Atmakuri K; Cascales E; Burton OT; Banta LM; Christie PJ (2007). Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. EMBO J. 26(10):2540-51. [PudMed:17505518]

(2) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]

(1) Mossey P; Hudacek A; Das A (2010). Agrobacterium tumefaciens type IV secretion protein VirB3 is an inner membrane protein and requires VirB4, VirB7, and VirB8 for stabilization. J Bacteriol. 192(11):2830-8. [PudMed:20348257]

This literature contains experimental investigation