| Effector Protein ID: 190014782 |
 T4SS name [ID] | VirB [15] |
| Gene symbol | virD2 |
| Locus tag | pTiBo148 |
| Strain | Agrobacterium tumefaciens plasmid Ti plasmid pTiBo542 (NC_010929) |
| Replicon | plasmid Ti plasmid pTiBo542 [GenBank: NC_010929] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 165626..166900 [+] |
| Product | type IV secretion system T-DNA border endonuclease VirD2 |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5' end of the resulting T-strand. VirD2 serves as a "pilot protein" to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | A5WY65 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 2.5e-48, Aligned region: 16..263 |
| Protein Sequence: 424 a.a. [Download] |
| >gi|190014782|ref|YP_001967546.1| VirD2 [Agrobacterium tumefaciens] MPDRAQVIIRIVPGGGTKTLQQIINQLEYLSRKGKLELQRSARHLDIPVPPDQIRELAQSWVTEAGIYDE SQSDDDRQQDLTTHIIVSFPAGTDQTAAYEASREWAAEMFGSGYGGGRYNYLTAYHVDRDHPHLHVVVNR RELLGQGWLKISRRHPQLNYDGLRKKMAEISLRHGIVLDATSRAERGIAERPITYAEYRRLERMQAQKIQ FEDTDFDETSPEEDRRDLSQSFDPFRSDASAGEPDRATRHDKQPLEPHARFQEPAGSSIKADARIRVPLE SERGAQPSASKIPVTGHFGIETSYVAEASVPKQSGNSDTSRPVTDVAMHTVERQQRSKRRHDEEAGPSGA NRKRLKAAQVDSEANVGEPDGRDDSNKAADPVSASIRTEQPEASPTCPRDRHDGELGERKRARGNRRDDG RGGT |
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| Nucleotide Sequence: 1275 bp [Download] |
| >gi|190014640|ref|NC_010929.1|:165626-166900 Agrobacterium tumefaciens Ti plasmid pTiBo542, complete sequence ATGCCCGATCGCGCTCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTTCAGCAGATAA TCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGAACTGCAGCGTTCAGCCCGGCATCTCGATAT TCCCGTTCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGTAAGCTTCCCCGCAGGTACCG ACCAAACCGCAGCTTATGAAGCAAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGG CCGCTATAACTATCTGACAGCCTACCACGTCGACCGCGATCATCCACATTTACATGTCGTGGTCAATCGT CGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTAC GGAAAAAGATGGCAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCGCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACATATGCTGAATATCGACGCCTTGAGCGGATGCAGGCTCAAAAGATTCAA TTCGAAGATACAGATTTTGATGAGACCTCGCCTGAGGAAGATCGTCGGGACCTCAGTCAATCGTTCGATC CATTTCGATCGGACGCATCTGCCGGCGAACCGGACCGTGCAACCCGACATGACAAACAACCGCTTGAACC GCACGCCCGTTTCCAGGAGCCCGCCGGCTCCAGCATCAAAGCCGACGCACGGATCCGCGTACCATTGGAG AGCGAGCGGGGTGCCCAACCATCCGCGTCCAAAATCCCTGTAACTGGGCATTTCGGGATTGAGACTTCGT ATGTCGCTGAAGCCAGCGTGCCCAAACAAAGCGGCAATTCCGATACTTCTCGCCCGGTGACTGACGTTGC CATGCACACAGTCGAGCGCCAGCAGCGATCAAAACGACGTCATGACGAGGAGGCAGGTCCGAGCGGAGCA AACCGTAAAAGATTGAAGGCCGCGCAAGTTGATTCCGAGGCAAATGTCGGTGAGCCCGACGGTCGCGATG ACAGCAACAAGGCGGCTGATCCGGTGTCTGCTTCCATCCGTACCGAGCAACCGGAAGCTTCTCCAACGTG TCCGCGTGACCGTCACGATGGAGAATTGGGAGAACGCAAACGTGCAAGAGGTAATCGTCGCGACGATGGG CGCGGGGGGACCTAG |