Detailed information NTG0999404

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABEG41_RS12890
• Genome accession: NZ_CP155795
• Coordinates: 2494398..2495015 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain D137
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2489398..2500015

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEG41_RS12860 (ABEG41_12860)	rpsT	2489707..2489973 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ABEG41_RS12865 (ABEG41_12865)	holA	2489988..2491031 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
ABEG41_RS12870 (ABEG41_12870)	-	2491071..2491220 (-)	150	WP_003229985.1	hypothetical protein	-
ABEG41_RS12875 (ABEG41_12875)	yqzM	2491261..2491395 (+)	135	WP_003229983.1	YqzM family protein	-
ABEG41_RS12880 (ABEG41_12880)	comEC	2491435..2493765 (-)	2331	WP_074794436.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABEG41_RS12885 (ABEG41_12885)	comEB	2493762..2494331 (-)	570	WP_015714288.1	ComE operon protein 2	-
ABEG41_RS12890 (ABEG41_12890)	comEA	2494398..2495015 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
ABEG41_RS12895 (ABEG41_12895)	comER	2495099..2495920 (+)	822	WP_015714289.1	late competence protein ComER	-
ABEG41_RS12900 (ABEG41_12900)	yqeM	2495986..2496729 (-)	744	WP_015714290.1	class I SAM-dependent methyltransferase	-
ABEG41_RS12905 (ABEG41_12905)	rsfS	2496726..2497082 (-)	357	WP_014480315.1	ribosome silencing factor	-
ABEG41_RS12910 (ABEG41_12910)	yqeK	2497100..2497660 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABEG41_RS12915 (ABEG41_12915)	nadD	2497650..2498219 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ABEG41_RS12920 (ABEG41_12920)	yhbY	2498231..2498521 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ABEG41_RS12925 (ABEG41_12925)	aroE	2498515..2499357 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=999404 ABEG41_RS12890 WP_004398514.1 2494398..2495015(-) (comEA) [Bacillus subtilis strain D137]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=999404 ABEG41_RS12890 WP_004398514.1 2494398..2495015(-) (comEA) [Bacillus subtilis strain D137]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39