Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ABC804_RS00565 Genome accession   NZ_CP155536
Coordinates   91380..92678 (+) Length   432 a.a.
NCBI ID   WP_000867612.1    Uniprot ID   A0A0E9GXK4
Organism   Streptococcus pneumoniae strain M264-3     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 86380..97678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC804_RS00535 (ABC804_00535) tsf 86847..87887 (+) 1041 WP_000808063.1 translation elongation factor Ts -
  ABC804_RS00540 (ABC804_00540) tnpB 88133..88456 (+) 324 WP_000973415.1 IS66 family insertion sequence element accessory protein TnpB -
  ABC804_RS00545 (ABC804_00545) - 88611..88898 (-) 288 Protein_98 transposase -
  ABC804_RS00550 (ABC804_00550) - 88880..89409 (+) 530 Protein_99 transposase -
  ABC804_RS00555 (ABC804_00555) cysK 89484..90404 (-) 921 WP_000029867.1 cysteine synthase A -
  ABC804_RS00560 (ABC804_00560) - 90689..91324 (-) 636 WP_000395739.1 YigZ family protein -
  ABC804_RS00565 (ABC804_00565) comFA/cflA 91380..92678 (+) 1299 WP_000867612.1 DEAD/DEAH box helicase Machinery gene
  ABC804_RS00570 (ABC804_00570) comFC/cflB 92675..93337 (+) 663 WP_000649971.1 ComF family protein Machinery gene
  ABC804_RS00575 (ABC804_00575) raiA 93417..93965 (+) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  ABC804_RS00580 (ABC804_00580) - 94101..96074 (+) 1974 WP_000715124.1 DHH family phosphoesterase -
  ABC804_RS00585 (ABC804_00585) rplI 96071..96523 (+) 453 WP_000864220.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49563.48 Da        Isoelectric Point: 8.4382

>NTDB_id=997387 ABC804_RS00565 WP_000867612.1 91380..92678(+) (comFA/cflA) [Streptococcus pneumoniae strain M264-3]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=997387 ABC804_RS00565 WP_000867612.1 91380..92678(+) (comFA/cflA) [Streptococcus pneumoniae strain M264-3]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E9GXK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment