Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AAHC66_RS22195 Genome accession   NZ_CP155078
Coordinates   4610609..4612624 (+) Length   671 a.a.
NCBI ID   WP_002742599.1    Uniprot ID   -
Organism   Microcystis aeruginosa PCC 7806     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4605609..4617624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHC66_RS22165 - 4605852..4606247 (-) 396 WP_002738951.1 PH domain-containing protein -
  AAHC66_RS22170 rnpA 4606237..4606599 (-) 363 WP_084989793.1 ribonuclease P protein component -
  AAHC66_RS22175 rpmH 4606626..4606763 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  AAHC66_RS22180 - 4606855..4608819 (+) 1965 WP_002742602.1 amylo-alpha-1,6-glucosidase -
  AAHC66_RS22185 - 4608910..4609551 (+) 642 WP_002742601.1 HAD-IA family hydrolase -
  AAHC66_RS22190 grpE 4609646..4610368 (-) 723 WP_002742600.1 nucleotide exchange factor GrpE -
  AAHC66_RS22195 pilB/pilB1 4610609..4612624 (+) 2016 WP_002742599.1 GspE/PulE family protein Machinery gene
  AAHC66_RS22200 pilT 4612777..4613883 (+) 1107 WP_002742598.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHC66_RS22205 - 4614024..4615238 (+) 1215 WP_036400185.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75292.54 Da        Isoelectric Point: 6.5258

>NTDB_id=996157 AAHC66_RS22195 WP_002742599.1 4610609..4612624(+) (pilB/pilB1) [Microcystis aeruginosa PCC 7806]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPTAILIAMVDPDNLAATDDINRILRPRELGLQRLV
IAGEDYERLLEKFYQAQSELEREKARLQKEKELEKLSDLTDIVGSLDLTSGVANDEVADDLGEGDANQAPVINLVNKIIA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKIAPAVTARFKIMADLDISERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDRNTLQIFRDLASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGININTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTVIEAALTGHLVLTTLHTNDAAGAIVRLD
KMGIEPFMISGSLLGILAQRLVRRVCTQCRVAYHPSQEELARFGLSAANEQEVTFYKANTLTMEEIQQARKQGNLCLKCA
GSGYKDRIGVYEVMQNSERIQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTLTDSGLEAELKAK
RKSGLICSGCRAELQPEWLDCPYCMRPRFSY

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=996157 AAHC66_RS22195 WP_002742599.1 4610609..4612624(+) (pilB/pilB1) [Microcystis aeruginosa PCC 7806]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTCGCCCTACGCAATTACTTTTCCCCTTTTGGTAACAAGTTAGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAGCAGGCCCTGGTGGAAACGCGGAAATCGGGACGATCGCTAGTAGAAATATTGC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAGTTGAGCGACGTGGATGGACTGGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCAACCGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGCGGCTACTGACGATATCAATCGTATTCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTGCCGGGGAAGATTATGAAAGATTACTGGAAAAATTCTACCAAGCCCAATCGGAACTAGAACGGGAGAAAGCCCGACT
ACAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAGTCTTGATTTGACCTCTGGTGTTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGATGCTAATCAAGCCCCCGTCATCAACCTTGTCAATAAAATTATAGCT
AAAGCTCTACAGGAAGGATCCTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATCGCCCCAGCGGTCACAGCCCGGTTTAAAATCATGGCCGATC
TCGATATTTCCGAACGTCGTCTTCCCCAGGATGGTAAAATTCGGCGGATGTATCAAGGACGAAAAGTAGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTAAGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGATCGAAATACTCTGCAAATTTTCCGGGACTTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CCACTGGTTCGGGAAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCCGGAATTAATATTAATACCGCC
GAAGACCCGATCGAATATTCTCTGCCAGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCCTGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAAGGAAACGGCTAAAACGGTGA
TCGAGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACTAACGATGCCGCAGGTGCGATCGTTCGTCTTGAT
AAAATGGGGATTGAACCATTTATGATTTCTGGTTCTCTGTTGGGAATCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAC
CCAGTGTCGGGTCGCCTATCACCCTAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGA
CTTTTTATAAGGCTAATACCCTAACTATGGAGGAAATTCAACAAGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTGCT
GGCAGCGGTTACAAAGATCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGAATACAGCAGTTAATTAACCAAGG
GGCGACAACCGATCGCATTAAGGAGGCGGCCGTTGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTAGAACGGGTGACACTGACCGATTCTGGTCTAGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCTGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGAGACC
GCGATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.508

98.659

0.715

Multiple sequence alignment