Detailed information NTG0995450

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAVB74_RS09985
• Genome accession: NZ_CP154920
• Coordinates: 1866219..1866836 (+)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain FUA2232
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1861219..1871836

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVB74_RS09950 (AAVB74_09950)	aroE	1861883..1862725 (+)	843	WP_038429328.1	shikimate dehydrogenase	-
AAVB74_RS09955 (AAVB74_09955)	yhbY	1862719..1863009 (+)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AAVB74_RS09960 (AAVB74_09960)	nadD	1863021..1863590 (+)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AAVB74_RS09965 (AAVB74_09965)	yqeK	1863580..1864140 (+)	561	WP_029317884.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAVB74_RS09970 (AAVB74_09970)	rsfS	1864158..1864508 (+)	351	WP_345806061.1	ribosome silencing factor	-
AAVB74_RS09975 (AAVB74_09975)	yqeM	1864505..1865248 (+)	744	WP_106073582.1	class I SAM-dependent methyltransferase	-
AAVB74_RS09980 (AAVB74_09980)	comER	1865314..1866135 (-)	822	WP_004398597.1	late competence protein ComER	-
AAVB74_RS09985 (AAVB74_09985)	comEA	1866219..1866836 (+)	618	WP_004398514.1	competence protein ComEA	Machinery gene
AAVB74_RS09990 (AAVB74_09990)	comEB	1866903..1867472 (+)	570	WP_015714288.1	ComE operon protein 2	-
AAVB74_RS09995 (AAVB74_09995)	comEC	1867469..1869799 (+)	2331	WP_345806062.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAVB74_RS10000 (AAVB74_10000)	yqzM	1869839..1869973 (-)	135	WP_003229983.1	YqzM family protein	-
AAVB74_RS10005 (AAVB74_10005)	-	1870014..1870163 (+)	150	WP_003229985.1	hypothetical protein	-
AAVB74_RS10010 (AAVB74_10010)	holA	1870203..1871246 (+)	1044	WP_345806063.1	DNA polymerase III subunit delta	-
AAVB74_RS10015 (AAVB74_10015)	rpsT	1871261..1871527 (-)	267	WP_003229989.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=995450 AAVB74_RS09985 WP_004398514.1 1866219..1866836(+) (comEA) [Bacillus subtilis strain FUA2232]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=995450 AAVB74_RS09985 WP_004398514.1 1866219..1866836(+) (comEA) [Bacillus subtilis strain FUA2232]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39