Detailed information NTG0994832

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAVC70_RS03135
• Genome accession: NZ_CP154898
• Coordinates: 439526..440140 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain Fad 97
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434526..445140

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVC70_RS03105 (AAVC70_03105)	rpsT	434826..435092 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AAVC70_RS03110 (AAVC70_03110)	holA	435109..436150 (-)	1042	Protein_505	DNA polymerase III subunit delta	-
AAVC70_RS03115 (AAVC70_03115)	-	436190..436344 (-)	155	Protein_506	hypothetical protein	-
AAVC70_RS03120 (AAVC70_03120)	-	436385..436519 (+)	135	WP_003152870.1	YqzM family protein	-
AAVC70_RS03125 (AAVC70_03125)	comEC	436538..438889 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAVC70_RS03130 (AAVC70_03130)	-	438890..439459 (-)	570	WP_003152868.1	ComE operon protein 2	-
AAVC70_RS03135 (AAVC70_03135)	comEA	439526..440140 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAVC70_RS03140 (AAVC70_03140)	comER	440199..441020 (+)	822	WP_013352943.1	late competence protein ComER	-
AAVC70_RS03145 (AAVC70_03145)	-	441089..441826 (-)	738	WP_013352944.1	class I SAM-dependent methyltransferase	-
AAVC70_RS03150 (AAVC70_03150)	rsfS	441823..442179 (-)	357	WP_003152864.1	ribosome silencing factor	-
AAVC70_RS03155 (AAVC70_03155)	yqeK	442197..442757 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAVC70_RS03160 (AAVC70_03160)	-	442747..443316 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
AAVC70_RS03165 (AAVC70_03165)	yhbY	443327..443617 (-)	291	WP_061573777.1	ribosome assembly RNA-binding protein YhbY	-
AAVC70_RS03170 (AAVC70_03170)	aroE	443611..444453 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=994832 AAVC70_RS03135 WP_013352942.1 439526..440140(-) (comEA) [Bacillus amyloliquefaciens strain Fad 97]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=994832 AAVC70_RS03135 WP_013352942.1 439526..440140(-) (comEA) [Bacillus amyloliquefaciens strain Fad 97]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363