Detailed information NTG0994800

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAVB98_RS16815
• Genome accession: NZ_CP154895
• Coordinates: 3204074..3204688 (+)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain Fad 77
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3199074..3209688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVB98_RS16780 (AAVB98_16780)	aroE	3199761..3200603 (+)	843	WP_013352947.1	shikimate dehydrogenase	-
AAVB98_RS16785 (AAVB98_16785)	yhbY	3200597..3200887 (+)	291	WP_061573777.1	ribosome assembly RNA-binding protein YhbY	-
AAVB98_RS16790 (AAVB98_16790)	-	3200898..3201467 (+)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
AAVB98_RS16795 (AAVB98_16795)	yqeK	3201457..3202017 (+)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAVB98_RS16800 (AAVB98_16800)	rsfS	3202035..3202391 (+)	357	WP_003152864.1	ribosome silencing factor	-
AAVB98_RS16805 (AAVB98_16805)	-	3202388..3203125 (+)	738	WP_013352944.1	class I SAM-dependent methyltransferase	-
AAVB98_RS16810 (AAVB98_16810)	comER	3203194..3204015 (-)	822	WP_013352943.1	late competence protein ComER	-
AAVB98_RS16815 (AAVB98_16815)	comEA	3204074..3204688 (+)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAVB98_RS16820 (AAVB98_16820)	-	3204755..3205324 (+)	570	WP_003152868.1	ComE operon protein 2	-
AAVB98_RS16825 (AAVB98_16825)	comEC	3205325..3207676 (+)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAVB98_RS16830 (AAVB98_16830)	-	3207695..3207829 (-)	135	WP_003152870.1	YqzM family protein	-
AAVB98_RS16835 (AAVB98_16835)	-	3207870..3208024 (+)	155	Protein_3252	hypothetical protein	-
AAVB98_RS16840 (AAVB98_16840)	holA	3208064..3209105 (+)	1042	Protein_3253	DNA polymerase III subunit delta	-
AAVB98_RS16845 (AAVB98_16845)	rpsT	3209122..3209388 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=994800 AAVB98_RS16815 WP_013352942.1 3204074..3204688(+) (comEA) [Bacillus amyloliquefaciens strain Fad 77]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=994800 AAVB98_RS16815 WP_013352942.1 3204074..3204688(+) (comEA) [Bacillus amyloliquefaciens strain Fad 77]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363