Detailed information NTG0993398

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: E0E14_RS08620
• Genome accession: NZ_LR027877
• Coordinates: 1716000..1717004 (-)
• Length: 334 a.a.
• NCBI ID: WP_001005767.1
• Uniprot ID: Q6GG63
• Organism: Staphylococcus aureus isolate BPH3244
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1711000..1722004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0E14_RS08605	yajC	1713532..1713792 (-)	261	WP_001160682.1	preprotein translocase subunit YajC	-
E0E14_RS08610	tgt	1713811..1714950 (-)	1140	WP_001112045.1	tRNA guanosine(34) transglycosylase Tgt	-
E0E14_RS08615	queA	1714973..1715998 (-)	1026	WP_001019172.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
E0E14_RS08620	ruvB	1716000..1717004 (-)	1005	WP_001005767.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
E0E14_RS08625	ruvA	1717036..1717638 (-)	603	WP_000271554.1	Holliday junction branch migration protein RuvA	Machinery gene
E0E14_RS08630	-	1717652..1718107 (-)	456	WP_000368902.1	ACT domain-containing protein	-
E0E14_RS08635	obgE	1718120..1719412 (-)	1293	WP_000496100.1	GTPase ObgE	-
E0E14_RS08640	rpmA	1719767..1720051 (-)	285	WP_000916187.1	50S ribosomal protein L27	-
E0E14_RS08645	-	1720063..1720383 (-)	321	WP_000633692.1	ribosomal-processing cysteine protease Prp	-
E0E14_RS08650	rplU	1720389..1720697 (-)	309	WP_000457385.1	50S ribosomal protein L21	-
E0E14_RS08655	mreD	1720864..1721394 (-)	531	WP_001259925.1	rod shape-determining protein MreD	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 37718.00 Da
• Isoelectric Point: 5.0865

>NTDB_id=993398 E0E14_RS08620 WP_001005767.1 1716000..1717004(-) (ruvB) [Staphylococcus aureus isolate BPH3244]
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERE

Nucleotide

• Length: 1005 bp

>NTDB_id=993398 E0E14_RS08620 WP_001005767.1 1716000..1717004(-) (ruvB) [Staphylococcus aureus isolate BPH3244]
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
TTTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAACGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGCGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (182-255)

• RuvB C-terminal winged helix domain (WHD_RuvB) (257-326)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (21-179)

3D structure

• AlphaFold DB: Q6GG63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	65.951	97.605	0.644
ruvB	Streptococcus pneumoniae TIGR4	58.182	98.802	0.575
ruvB	Streptococcus pneumoniae R6	57.879	98.802	0.572
ruvB	Streptococcus pneumoniae D39	57.879	98.802	0.572
ruvB	Helicobacter pylori 26695	56.481	97.006	0.548
ruvB	Synechocystis sp. PCC 6803	53.75	95.808	0.515