Detailed information NTG0992334

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KKKWG1_RS09965
• Genome accession: NZ_LN869922
• Coordinates: 1921038..1922318 (-)
• Length: 426 a.a.
• NCBI ID: WP_003787551.1
• Uniprot ID: F5S877
• Organism: Kingella kingae strain KWG1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1916038..1927318

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KKKWG1_RS09940 (KKKWG1_2024)	folD	1917056..1917907 (+)	852	WP_003791096.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-
KKKWG1_RS09945 (KKKWG1_2025)	-	1918050..1919438 (+)	1389	WP_060537189.1	GntP family permease	-
KKKWG1_RS09950 (KKKWG1_2026)	-	1919563..1920072 (+)	510	WP_038317696.1	gluconokinase	-
KKKWG1_RS09955 (KKKWG1_2027)	-	1920143..1920520 (-)	378	WP_038317695.1	diacylglycerol kinase	-
KKKWG1_RS09960 (KKKWG1_2028)	-	1920517..1920960 (-)	444	WP_019389590.1	peroxiredoxin	-
KKKWG1_RS09965 (KKKWG1_2029)	pilU	1921038..1922318 (-)	1281	WP_003787551.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KKKWG1_RS09970 (KKKWG1_2030)	pilT	1922338..1923378 (-)	1041	WP_003791105.1	type IV pilus twitching motility protein PilT	Machinery gene
KKKWG1_RS09975 (KKKWG1_2031)	pseC	1923814..1924971 (-)	1158	WP_019389589.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase	-
KKKWG1_RS09980 (KKKWG1_2032)	-	1925084..1926259 (-)	1176	WP_003787543.1	alpha-hydroxy acid oxidase	-

Sequence

Protein

• Length: 426 a.a.
• Molecular weight: 47321.06 Da
• Isoelectric Point: 6.2389

>NTDB_id=992334 KKKWG1_RS09965 WP_003787551.1 1921038..1922318(-) (pilU) [Kingella kingae strain KWG1]
MAEQDITSLLEGFVNNYKVTNDAAKPAAPAGRAVPASAQATATASEIAPAKKSGLHPLLEKMAAECEKRGASDIFISAGF
PPSFKINGELTPVPLKPLTQQDTQILVYSTMSPDQRARFDHELELNYSVSSSEGIRFRVNSYHEQGRMGMVWRRITTKIL
TVDDLQLPQGLKDLAMQPRGLLILAGATGSGKSTSMAAMLDWRNKHAAGHIVTIEDPVEYLHKPIKSIFTQRELGVDTNS
WSMAVQSAMRQAPDVVCVGEVRNEHTMEYALQLAQTGHLCIFTIHANNAVQTIERIMNFYPEERHQQVLMDLALNTVGII
GQRLARLKGGGRTAIVDLLINSPAMQDHIFKGELMEAMSLMNRSETDGMQTFDQDLFRLYTEDKITYEEALRQAESVNDL
RLKIKLWEEGNAKDHIFERVGGLSLQ

Nucleotide

• Length: 1281 bp

>NTDB_id=992334 KKKWG1_RS09965 WP_003787551.1 1921038..1922318(-) (pilU) [Kingella kingae strain KWG1]
ATGGCAGAACAAGACATCACTTCCCTACTTGAAGGTTTTGTAAACAATTACAAAGTAACCAACGATGCCGCCAAGCCTGC
TGCACCAGCAGGACGAGCCGTGCCTGCATCGGCACAAGCCACGGCAACGGCTTCGGAAATTGCGCCAGCCAAAAAATCGG
GCTTACACCCTTTGTTGGAAAAAATGGCTGCCGAATGTGAAAAACGCGGTGCATCGGATATTTTTATCAGCGCAGGTTTT
CCGCCATCATTCAAAATCAATGGTGAATTAACGCCTGTTCCGCTCAAACCGCTCACGCAGCAAGACACGCAAATTTTGGT
GTATTCCACCATGTCGCCAGACCAACGTGCGCGTTTTGACCACGAATTGGAATTGAACTACTCCGTGTCGTCTAGCGAGG
GCATTCGCTTTCGTGTGAACTCGTATCACGAGCAAGGTCGCATGGGCATGGTGTGGCGTCGCATCACCACCAAAATCTTA
ACGGTTGATGATTTGCAGTTGCCACAAGGTTTAAAAGACTTGGCGATGCAGCCGCGCGGTTTGTTGATTTTGGCAGGCGC
AACGGGTTCGGGTAAATCTACGTCTATGGCGGCGATGCTGGACTGGCGCAACAAACACGCCGCAGGTCATATTGTGACCA
TTGAAGACCCTGTCGAATATTTGCATAAGCCAATCAAGAGTATTTTCACGCAACGCGAATTGGGCGTGGATACGAATTCT
TGGTCAATGGCGGTGCAATCGGCAATGCGTCAAGCACCAGACGTGGTTTGTGTGGGTGAGGTGCGTAACGAACACACCAT
GGAATACGCTTTGCAACTGGCGCAAACAGGTCACTTGTGTATCTTTACCATTCACGCGAACAATGCCGTGCAAACCATTG
AGCGTATTATGAACTTCTATCCAGAAGAGCGTCATCAACAAGTATTAATGGACTTGGCGTTGAACACAGTGGGCATTATT
GGTCAGCGTTTGGCGCGATTGAAAGGCGGCGGACGTACGGCGATTGTGGATTTGTTGATTAACAGCCCTGCGATGCAAGA
CCACATTTTCAAAGGCGAGTTGATGGAAGCGATGTCGTTGATGAATCGTTCGGAAACAGACGGCATGCAAACGTTTGACC
AAGACTTGTTCCGCTTGTACACCGAAGACAAAATCACTTACGAAGAAGCCTTGCGCCAAGCTGAATCGGTAAACGACTTG
CGCTTGAAAATCAAATTGTGGGAAGAGGGTAATGCCAAAGACCACATCTTTGAGCGCGTGGGCGGTTTGAGCTTGCAATA
A

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (96-326)

3D structure

• AlphaFold DB: F5S877

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	47.696	86.62	0.413
pilU	Acinetobacter baylyi ADP1	49.711	81.221	0.404
pilU	Vibrio cholerae strain A1552	46.429	78.873	0.366