Detailed information NTG0992227

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHU43_RS21770
• Genome accession: NZ_CP153807
• Coordinates: 4454105..4454905 (+)
• Length: 266 a.a.
• NCBI ID: WP_023297075.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain KP_NORM_BLD_114309
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4449105..4459905

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHU43_RS21735 (AAHU43_21720)	gspH	4449258..4449773 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHU43_RS21740 (AAHU43_21725)	gspI	4449770..4450135 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHU43_RS21745 (AAHU43_21730)	gspJ	4450132..4450728 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
AAHU43_RS21750 (AAHU43_21735)	gspK	4450725..4451705 (+)	981	WP_023297078.1	type II secretion system minor pseudopilin GspK	-
AAHU43_RS21755 (AAHU43_21740)	gspL	4451698..4452894 (+)	1197	WP_023339600.1	type II secretion system protein GspL	-
AAHU43_RS21760 (AAHU43_21745)	-	4452887..4453372 (+)	486	WP_008807382.1	type II secretion system protein M	-
AAHU43_RS21765 (AAHU43_21750)	-	4453362..4454108 (+)	747	WP_023297076.1	type II secretion system protein N	-
AAHU43_RS21770 (AAHU43_21755)	pilD	4454105..4454905 (+)	801	WP_023297075.1	A24 family peptidase	Machinery gene
AAHU43_RS21775 (AAHU43_21760)	hrpB	4454952..4457381 (-)	2430	WP_023297074.1	ATP-dependent helicase HrpB	-
AAHU43_RS21780 (AAHU43_21765)	thpR	4457454..4457990 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHU43_RS21785 (AAHU43_21770)	sfsA	4457990..4458706 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHU43_RS21790 (AAHU43_21775)	dksA	4458869..4459324 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29491.18 Da
• Isoelectric Point: 8.4742

>NTDB_id=992227 AAHU43_RS21770 WP_023297075.1 4454105..4454905(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_114309]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIEWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=992227 AAHU43_RS21770 WP_023297075.1 4454105..4454905(+) (pilD) [Klebsiella variicola subsp. variicola strain KP_NORM_BLD_114309]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGAGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425