Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | ACGHAS_RS20275 | Genome accession | NZ_AP035801 |
| Coordinates | 4163950..4164390 (+) | Length | 146 a.a. |
| NCBI ID | WP_000360915.1 | Uniprot ID | A0A0H2V436 |
| Organism | Escherichia coli strain E. coli JCM:5491 | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
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Genomic Context
Location: 4158950..4169390
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACGHAS_RS20255 (ReJCM5491_38280) | aroP | 4159950..4161320 (+) | 1371 | WP_000399017.1 | aromatic amino acid transporter AroP | - |
| ACGHAS_RS20260 (ReJCM5491_38290) | ampE | 4161363..4162217 (-) | 855 | WP_000172013.1 | beta-lactamase regulator AmpE | - |
| ACGHAS_RS20265 (ReJCM5491_38300) | ampD | 4162214..4162765 (-) | 552 | WP_000923726.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| ACGHAS_RS20270 (ReJCM5491_38310) | nadC | 4162853..4163746 (+) | 894 | WP_001135168.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| ACGHAS_RS20275 (ReJCM5491_38320) | pilA | 4163950..4164390 (+) | 441 | WP_000360915.1 | prepilin peptidase-dependent pilin | Machinery gene |
| ACGHAS_RS20280 (ReJCM5491_38330) | pilB | 4164400..4165785 (+) | 1386 | WP_001025180.1 | type II secretion system protein GspE | Machinery gene |
| ACGHAS_RS20285 (ReJCM5491_38340) | hofC | 4165775..4166977 (+) | 1203 | WP_000157242.1 | protein transport protein HofC | - |
| ACGHAS_RS20290 (ReJCM5491_38350) | guaC | 4167012..4168055 (-) | 1044 | WP_001217338.1 | GMP reductase | - |
| ACGHAS_RS20295 | - | 4168211..4168255 (-) | 45 | WP_120795372.1 | protein YacM | - |
| ACGHAS_RS20300 (ReJCM5491_38360) | coaE | 4168280..4168900 (+) | 621 | WP_001269525.1 | dephospho-CoA kinase | - |
Sequence
Protein
Download Length: 146 a.a. Molecular weight: 15607.77 Da Isoelectric Point: 4.5237
>NTDB_id=99141 ACGHAS_RS20275 WP_000360915.1 4163950..4164390(+) (pilA) [Escherichia coli strain E. coli JCM:5491]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN
Nucleotide
Download Length: 441 bp
>NTDB_id=99141 ACGHAS_RS20275 WP_000360915.1 4163950..4164390(+) (pilA) [Escherichia coli strain E. coli JCM:5491]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Haemophilus influenzae 86-028NP |
43.089 |
84.247 |
0.363 |