Detailed information NTG0990428

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY52_RS22625
• Genome accession: NZ_CP153119
• Coordinates: 4618933..4619733 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain T7-493
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4613933..4624733

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY52_RS22590 (AAHY52_22560)	gspH	4614086..4614601 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHY52_RS22595 (AAHY52_22565)	gspI	4614598..4614963 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY52_RS22600 (AAHY52_22570)	gspJ	4614960..4615556 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHY52_RS22605 (AAHY52_22575)	gspK	4615553..4616533 (+)	981	WP_077269942.1	type II secretion system minor pseudopilin GspK	-
AAHY52_RS22610 (AAHY52_22580)	gspL	4616526..4617722 (+)	1197	WP_032740434.1	type II secretion system protein GspL	-
AAHY52_RS22615 (AAHY52_22585)	-	4617715..4618200 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHY52_RS22620 (AAHY52_22590)	-	4618190..4618936 (+)	747	WP_072124978.1	type II secretion system protein N	-
AAHY52_RS22625 (AAHY52_22595)	pilD	4618933..4619733 (+)	801	WP_023322076.1	A24 family peptidase	Machinery gene
AAHY52_RS22630 (AAHY52_22600)	hrpB	4619780..4622209 (-)	2430	WP_032732528.1	ATP-dependent helicase HrpB	-
AAHY52_RS22635 (AAHY52_22605)	thpR	4622282..4622818 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY52_RS22640 (AAHY52_22610)	sfsA	4622818..4623534 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHY52_RS22645 (AAHY52_22615)	dksA	4623697..4624152 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=990428 AAHY52_RS22625 WP_023322076.1 4618933..4619733(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-493]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990428 AAHY52_RS22625 WP_023322076.1 4618933..4619733(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-493]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425