Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WN937_RS13560 Genome accession   NZ_CP152363
Coordinates   2875352..2875825 (+) Length   157 a.a.
NCBI ID   WP_069536420.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain vp-HL-201910     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2870352..2880825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN937_RS13545 (WN937_13545) pdhR 2872385..2873152 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WN937_RS13550 (WN937_13550) ampD 2873558..2874109 (-) 552 WP_025554880.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WN937_RS13555 (WN937_13555) nadC 2874202..2875089 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WN937_RS13560 (WN937_13560) pilA 2875352..2875825 (+) 474 WP_069536420.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WN937_RS13565 (WN937_13565) pilB 2875831..2877516 (+) 1686 WP_069536419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WN937_RS13570 (WN937_13570) pilC 2877540..2878763 (+) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  WN937_RS13575 (WN937_13575) pilD 2878828..2879697 (+) 870 WP_025533026.1 A24 family peptidase Machinery gene
  WN937_RS13580 (WN937_13580) coaE 2879698..2880312 (+) 615 WP_005480887.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16666.33 Da        Isoelectric Point: 9.1138

>NTDB_id=988670 WN937_RS13560 WP_069536420.1 2875352..2875825(+) (pilA) [Vibrio parahaemolyticus strain vp-HL-201910]
MKHRKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKKE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADQVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=988670 WN937_RS13560 WP_069536420.1 2875352..2875825(+) (pilA) [Vibrio parahaemolyticus strain vp-HL-201910]
ATGAAACACCGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGATTATCGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGAAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCCGCAGCTGATCAAGTTAAGGGACCTATTGCTAAAGGCGAACAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.051

100

0.561
  pilA Haemophilus influenzae Rd KW20

42.361

91.72

0.389
  pilA Haemophilus influenzae 86-028NP

41.379

92.357

0.382

Multiple sequence alignment