Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AAEU35_RS01940 Genome accession   NZ_CP152294
Coordinates   413795..415117 (+) Length   440 a.a.
NCBI ID   WP_058223060.1    Uniprot ID   -
Organism   Lactococcus lactis strain Q13     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408795..420117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEU35_RS01915 - 409611..410258 (-) 648 WP_058223056.1 GIY-YIG nuclease family protein -
  AAEU35_RS01920 - 410550..411296 (-) 747 WP_058223057.1 bifunctional metallophosphatase/5'-nucleotidase -
  AAEU35_RS01925 - 411411..412430 (-) 1020 WP_012897709.1 DUF475 domain-containing protein -
  AAEU35_RS01930 - 412478..412975 (-) 498 WP_023189340.1 VanZ family protein -
  AAEU35_RS01935 - 413109..413738 (-) 630 WP_003130660.1 YigZ family protein -
  AAEU35_RS01940 comFA 413795..415117 (+) 1323 WP_058223060.1 DEAD/DEAH box helicase Machinery gene
  AAEU35_RS01945 comFC 415360..415764 (+) 405 WP_021722514.1 ComF family protein Machinery gene
  AAEU35_RS01950 - 415797..416375 (-) 579 WP_023189343.1 nucleotidyltransferase family protein -
  AAEU35_RS01955 - 416389..416844 (-) 456 WP_003130653.1 diacylglycerol kinase -
  AAEU35_RS01960 ybeY 416828..417316 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  AAEU35_RS01965 - 417468..417947 (-) 480 WP_058223061.1 NUDIX hydrolase -
  AAEU35_RS01970 - 418005..418973 (-) 969 WP_003130649.1 PhoH family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50300.85 Da        Isoelectric Point: 10.1066

>NTDB_id=988166 AAEU35_RS01940 WP_058223060.1 413795..415117(+) (comFA) [Lactococcus lactis strain Q13]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHHQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLVGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=988166 AAEU35_RS01940 WP_058223060.1 413795..415117(+) (comFA) [Lactococcus lactis strain Q13]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
ATCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGTAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

73.75

90.909

0.67
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38

Multiple sequence alignment