Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AAB158_RS17230 Genome accession   NZ_CP152291
Coordinates   3601457..3602533 (-) Length   358 a.a.
NCBI ID   WP_342615850.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain IMGN5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596457..3607533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAB158_RS17190 (AAB158_17190) - 3597322..3598122 (+) 801 WP_192205487.1 YqhG family protein -
  AAB158_RS17195 (AAB158_17195) - 3598166..3598351 (-) 186 WP_063233067.1 YqzE family protein -
  AAB158_RS17200 (AAB158_17200) comGG 3598468..3598854 (-) 387 WP_192205490.1 competence type IV pilus minor pilin ComGG -
  AAB158_RS17205 (AAB158_17205) comGF 3598847..3599326 (-) 480 WP_192208338.1 competence type IV pilus minor pilin ComGF -
  AAB158_RS17210 (AAB158_17210) - 3599274..3599612 (-) 339 WP_192205492.1 hypothetical protein -
  AAB158_RS17215 (AAB158_17215) comGD 3599599..3600042 (-) 444 WP_192205494.1 competence type IV pilus minor pilin ComGD -
  AAB158_RS17220 (AAB158_17220) comGC 3600050..3600361 (-) 312 WP_342614152.1 competence type IV pilus major pilin ComGC -
  AAB158_RS17225 (AAB158_17225) comGB 3600439..3601476 (-) 1038 WP_342614153.1 competence type IV pilus assembly protein ComGB -
  AAB158_RS17230 (AAB158_17230) comGA 3601457..3602533 (-) 1077 WP_342615850.1 competence type IV pilus ATPase ComGA Machinery gene
  AAB158_RS17235 (AAB158_17235) - 3602635..3603015 (-) 381 WP_192205498.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AAB158_RS17240 (AAB158_17240) - 3603231..3603926 (+) 696 WP_192205500.1 helix-turn-helix domain-containing protein -
  AAB158_RS17245 (AAB158_17245) - 3604012..3604254 (+) 243 WP_076365317.1 DUF2626 domain-containing protein -
  AAB158_RS17250 (AAB158_17250) - 3604310..3605407 (-) 1098 WP_342614154.1 SAM-dependent methyltransferase -
  AAB158_RS17255 (AAB158_17255) - 3605804..3606430 (-) 627 WP_192205504.1 MBL fold metallo-hydrolase -
  AAB158_RS17260 (AAB158_17260) - 3606668..3606841 (+) 174 WP_192205506.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39781.39 Da        Isoelectric Point: 8.1659

>NTDB_id=988109 AAB158_RS17230 WP_342615850.1 3601457..3602533(-) (comGA) [Peribacillus frigoritolerans strain IMGN5]
MISIEKTAEKILTRAVQESASDIHIFFRREGPLIQFRIDNKLVPKETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
IHLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPSTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
ANEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLVLTTMH
TRDAQGAISRLLEFGVSLLEVEQSLIGVTAQRLVELRCPPCKGDCALPCKMTARNKRASVYELLYGKSLAEVLGIMVDEK
GKATVSYRQLKDEIGKAVAMGYVDSEEYERLVYDETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=988109 AAB158_RS17230 WP_342615850.1 3601457..3602533(-) (comGA) [Peribacillus frigoritolerans strain IMGN5]
TTGATATCGATTGAAAAGACCGCAGAAAAAATATTGACCCGTGCTGTCCAGGAATCGGCATCGGATATCCACATTTTTTT
TCGAAGGGAGGGACCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCAAAGGAAACCTTATCATTCTTTGAAGCAG
AGAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAAAGTGGTGCCATCACC
ATCCATTTGGCCAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATCCCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAGCACCGTTCAAAAATTGATTGCGCTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGAAAAACCACGACACTATATTCCCTGCTTCACCAT
GCCAATGAGATGATCAATCGAAATATTATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAAGTATTGCAGGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCTGTCGGTCTAAAAGCTGTTCTGAGGCATGACCCAGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACCGCCAAGATCGCAGTGCGTGCCGCATTGACCGGTCATTTGGTACTTACAACCATGCAT
ACAAGGGACGCCCAGGGCGCCATCTCCAGGTTACTGGAATTTGGTGTCAGCTTGCTTGAGGTTGAGCAGAGTTTGATTGG
CGTGACAGCACAGCGGCTGGTTGAATTGCGATGTCCTCCATGTAAAGGGGACTGTGCTTTACCTTGCAAAATGACTGCCA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGTAAAAGCCTAGCTGAGGTTCTCGGGATAATGGTAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTGAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGATTCCGAGGA
ATATGAACGGCTGGTATATGATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.303

99.721

0.561
  pilB Haemophilus influenzae 86-028NP

39.368

97.207

0.383
  pilB Glaesserella parasuis strain SC1401

40.476

93.855

0.38
  pilB Haemophilus influenzae Rd KW20

37.931

97.207

0.369

Multiple sequence alignment