Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYE63_RS18995 Genome accession   NZ_CP152053
Coordinates   3898477..3899553 (-) Length   358 a.a.
NCBI ID   WP_342605825.1    Uniprot ID   -
Organism   Peribacillus sp. FSL E2-0159     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3893477..3904553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE63_RS18955 (NYE63_18950) - 3894340..3895137 (+) 798 WP_342602278.1 YqhG family protein -
  NYE63_RS18960 (NYE63_18955) - 3895184..3895369 (-) 186 WP_063233067.1 YqzE family protein -
  NYE63_RS18965 (NYE63_18960) comGG 3895488..3895874 (-) 387 WP_342602279.1 competence type IV pilus minor pilin ComGG -
  NYE63_RS18970 (NYE63_18965) comGF 3895867..3896346 (-) 480 WP_342602280.1 competence type IV pilus minor pilin ComGF -
  NYE63_RS18975 (NYE63_18970) - 3896294..3896632 (-) 339 WP_342602281.1 hypothetical protein -
  NYE63_RS18980 (NYE63_18975) comGD 3896619..3897062 (-) 444 WP_342602282.1 competence type IV pilus minor pilin ComGD -
  NYE63_RS18985 (NYE63_18980) comGC 3897070..3897381 (-) 312 WP_342605824.1 competence type IV pilus major pilin ComGC -
  NYE63_RS18990 (NYE63_18985) comGB 3897459..3898493 (-) 1035 WP_342602283.1 competence type IV pilus assembly protein ComGB -
  NYE63_RS18995 (NYE63_18990) comGA 3898477..3899553 (-) 1077 WP_342605825.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE63_RS19000 (NYE63_18995) - 3899658..3900038 (-) 381 WP_342602284.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYE63_RS19005 (NYE63_19000) - 3900254..3900955 (+) 702 WP_342602285.1 helix-turn-helix domain-containing protein -
  NYE63_RS19010 (NYE63_19005) - 3901041..3901283 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  NYE63_RS19015 (NYE63_19010) - 3901337..3902437 (-) 1101 WP_342602286.1 SAM-dependent methyltransferase -
  NYE63_RS19020 (NYE63_19015) - 3902793..3903419 (-) 627 WP_342602287.1 MBL fold metallo-hydrolase -
  NYE63_RS19025 (NYE63_19020) - 3903652..3903825 (+) 174 WP_342602288.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39793.45 Da        Isoelectric Point: 9.0151

>NTDB_id=987020 NYE63_RS18995 WP_342605825.1 3898477..3899553(-) (comGA) [Peribacillus sp. FSL E2-0159]
MISIEKTAEKILTRAVQESASDIHIFFRKEGPLIQFRIDNKLVPKETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPSTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSQKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEIRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQSLIGVTAQRLVELRCLPCKGDCDLGCKMTARNKRASVYELLYGKSLADVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDSGEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=987020 NYE63_RS18995 WP_342605825.1 3898477..3899553(-) (comGA) [Peribacillus sp. FSL E2-0159]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTGACCCGTGCTGTACAGGAATCGGCATCGGATATCCACATTTTTTT
TCGCAAGGAGGGTCCTCTCATCCAATTCAGGATAGATAATAAGCTTGTTCCAAAAGAAACATTATCATTCTTTGAAGCAG
AGAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCCAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCGACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTCGAACAGTTATCCTTATTTCCAAGCACCGTTCAAAAATTGATTGCTCTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGAAAAACCACGACACTATATTCCCTGCTTCACCAT
GCGCATGAGATGATCAATCGAAATATTATTACACTTGAAGATCCCATTGAAAATGTATCCCAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGGATTACGTATTCTGTTGGTCTAAAAGCTGTCCTGAGGCATGACCCTGACGTGATCATGGTTG
GGGAAATCAGGGATGCAGAAACTGCAAAAATCGCAGTGCGTGCCGCATTAACCGGTCATTTGATACTAACAACCATGCAT
ACAAGGGACGCCCAGGGCGCCATCTCCAGGTTACTGGAGTTTGGTGTCAGCTTGCTTGAGGTTGAACAGAGTTTAATTGG
CGTGACAGCACAGCGGCTGGTTGAGTTGCGATGTCTTCCATGTAAAGGGGACTGTGATTTAGGTTGCAAAATGACTGCCA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGCCTGGCTGATGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTGAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGATTCCGGGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

39.316

98.045

0.385
  pilB Glaesserella parasuis strain SC1401

38.776

95.81

0.372
  pilB Haemophilus influenzae Rd KW20

37.892

98.045

0.372

Multiple sequence alignment