Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI46_RS11425 Genome accession   NZ_CP152042
Coordinates   2224002..2225072 (-) Length   356 a.a.
NCBI ID   WP_260774219.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219002..2230072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS11375 (MHI46_11375) - 2219095..2219667 (-) 573 WP_047202287.1 signal peptidase I -
  MHI46_RS11380 (MHI46_11380) tapA 2219719..2220246 (-) 528 WP_059374880.1 amyloid fiber anchoring/assembly protein TapA -
  MHI46_RS11385 (MHI46_11385) - 2220546..2220824 (+) 279 WP_231120214.1 DUF3889 domain-containing protein -
  MHI46_RS11390 (MHI46_11390) - 2220858..2221052 (-) 195 WP_024718846.1 YqzE family protein -
  MHI46_RS11395 (MHI46_11395) comGG 2221112..2221495 (-) 384 WP_260774208.1 competence type IV pilus minor pilin ComGG -
  MHI46_RS11400 (MHI46_11400) comGF 2221492..2222022 (-) 531 WP_260774210.1 competence type IV pilus minor pilin ComGF -
  MHI46_RS11405 (MHI46_11405) comGE 2221931..2222248 (-) 318 WP_260774211.1 competence type IV pilus minor pilin ComGE -
  MHI46_RS11410 (MHI46_11410) comGD 2222232..2222678 (-) 447 WP_260774213.1 competence type IV pilus minor pilin ComGD -
  MHI46_RS11415 (MHI46_11415) comGC 2222671..2222964 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI46_RS11420 (MHI46_11420) comGB 2222981..2224021 (-) 1041 WP_260774217.1 competence type IV pilus assembly protein ComGB -
  MHI46_RS11425 (MHI46_11425) comGA 2224002..2225072 (-) 1071 WP_260774219.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI46_RS11430 (MHI46_11430) - 2225250..2226125 (-) 876 WP_047202296.1 STAS domain-containing protein -
  MHI46_RS11440 (MHI46_11440) - 2226350..2226730 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI46_RS11445 (MHI46_11445) - 2226944..2227186 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI46_RS11450 (MHI46_11450) - 2227225..2227863 (-) 639 WP_064497601.1 MBL fold metallo-hydrolase -
  MHI46_RS11455 (MHI46_11455) - 2228021..2228194 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI46_RS11460 (MHI46_11460) - 2228219..2228533 (-) 315 WP_034319597.1 MTH1187 family thiamine-binding protein -
  MHI46_RS11465 (MHI46_11465) - 2228610..2229863 (-) 1254 WP_260774229.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40382.92 Da        Isoelectric Point: 9.4005

>NTDB_id=986492 MHI46_RS11425 WP_260774219.1 2224002..2225072(-) (comGA) [Bacillus sp. FSL K6-1000]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEALVSFRVDSDLIQQRTIDKKTGERLIAHFKFLSSMDIGEKRRPQNGSLA
VVLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGEICQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986492 MHI46_RS11425 WP_260774219.1 2224002..2225072(-) (comGA) [Bacillus sp. FSL K6-1000]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTCCC
AAAAGAAAAGGAAGCGTTGGTATCTTTCCGCGTAGACTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAACCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGGTCATTAGCT
GTGGTGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACACCTGTCACTATTTCCGAAAGCTTCAGCCAGGTTATTGTCATTTTTGA
AGCATTCGCATGGGCTTATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACGCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAATATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649

Multiple sequence alignment