Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHI46_RS11415 Genome accession   NZ_CP152042
Coordinates   2222671..2222964 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus sp. FSL K6-1000     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217671..2227964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS11365 (MHI46_11365) sinR 2217834..2218169 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  MHI46_RS11370 (MHI46_11370) - 2218237..2219037 (-) 801 WP_039179929.1 TasA family protein -
  MHI46_RS11375 (MHI46_11375) - 2219095..2219667 (-) 573 WP_047202287.1 signal peptidase I -
  MHI46_RS11380 (MHI46_11380) tapA 2219719..2220246 (-) 528 WP_059374880.1 amyloid fiber anchoring/assembly protein TapA -
  MHI46_RS11385 (MHI46_11385) - 2220546..2220824 (+) 279 WP_231120214.1 DUF3889 domain-containing protein -
  MHI46_RS11390 (MHI46_11390) - 2220858..2221052 (-) 195 WP_024718846.1 YqzE family protein -
  MHI46_RS11395 (MHI46_11395) comGG 2221112..2221495 (-) 384 WP_260774208.1 competence type IV pilus minor pilin ComGG -
  MHI46_RS11400 (MHI46_11400) comGF 2221492..2222022 (-) 531 WP_260774210.1 competence type IV pilus minor pilin ComGF -
  MHI46_RS11405 (MHI46_11405) comGE 2221931..2222248 (-) 318 WP_260774211.1 competence type IV pilus minor pilin ComGE -
  MHI46_RS11410 (MHI46_11410) comGD 2222232..2222678 (-) 447 WP_260774213.1 competence type IV pilus minor pilin ComGD -
  MHI46_RS11415 (MHI46_11415) comGC 2222671..2222964 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI46_RS11420 (MHI46_11420) comGB 2222981..2224021 (-) 1041 WP_260774217.1 competence type IV pilus assembly protein ComGB -
  MHI46_RS11425 (MHI46_11425) comGA 2224002..2225072 (-) 1071 WP_260774219.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI46_RS11430 (MHI46_11430) - 2225250..2226125 (-) 876 WP_047202296.1 STAS domain-containing protein -
  MHI46_RS11440 (MHI46_11440) - 2226350..2226730 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI46_RS11445 (MHI46_11445) - 2226944..2227186 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI46_RS11450 (MHI46_11450) - 2227225..2227863 (-) 639 WP_064497601.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=986491 MHI46_RS11415 WP_024422977.1 2222671..2222964(-) (comGC) [Bacillus sp. FSL K6-1000]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=986491 MHI46_RS11415 WP_024422977.1 2222671..2222964(-) (comGC) [Bacillus sp. FSL K6-1000]
ATGAATGATAAAGGGTTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCAATTCAAGCAAAGGGCTGTGAAGGGCTCAAATCAATGGTCAGCACACAAGTGATGG
CGTATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAGTTAGAAAAAGAAGGTTATGTTAAAAAGGGATTAACC
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.222

92.784

0.392
  comGC Staphylococcus aureus N315

42.222

92.784

0.392

Multiple sequence alignment