Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYE42_RS12785 Genome accession   NZ_CP152036
Coordinates   2619845..2620915 (-) Length   356 a.a.
NCBI ID   WP_342496986.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2865     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614845..2625915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE42_RS12735 (NYE42_12715) sipW 2614905..2615489 (-) 585 WP_015240205.1 signal peptidase I SipW -
  NYE42_RS12740 (NYE42_12720) tapA 2615461..2616132 (-) 672 WP_095352946.1 amyloid fiber anchoring/assembly protein TapA -
  NYE42_RS12745 (NYE42_12725) - 2616391..2616720 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NYE42_RS12750 (NYE42_12730) - 2616760..2616939 (-) 180 WP_003153093.1 YqzE family protein -
  NYE42_RS12755 (NYE42_12735) comGG 2616996..2617373 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYE42_RS12760 (NYE42_12740) comGF 2617374..2617874 (-) 501 WP_260854721.1 competence type IV pilus minor pilin ComGF -
  NYE42_RS12765 (NYE42_12745) comGE 2617783..2618097 (-) 315 WP_352426500.1 competence type IV pilus minor pilin ComGE -
  NYE42_RS12770 (NYE42_12750) comGD 2618081..2618518 (-) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE42_RS12775 (NYE42_12755) comGC 2618508..2618816 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE42_RS12780 (NYE42_12760) comGB 2618821..2619858 (-) 1038 WP_342496985.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE42_RS12785 (NYE42_12765) comGA 2619845..2620915 (-) 1071 WP_342496986.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE42_RS12790 (NYE42_12770) - 2621108..2622058 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  NYE42_RS12795 (NYE42_12775) - 2622204..2623505 (+) 1302 WP_012117986.1 hemolysin family protein -
  NYE42_RS12800 (NYE42_12780) - 2623543..2624373 (-) 831 WP_352426501.1 STAS domain-containing protein -
  NYE42_RS12810 (NYE42_12790) - 2624580..2624960 (-) 381 WP_064115382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYE42_RS12815 (NYE42_12795) - 2625197..2625442 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40559.94 Da        Isoelectric Point: 9.4729

>NTDB_id=986143 NYE42_RS12785 WP_342496986.1 2619845..2620915(-) (comGA) [Bacillus sp. FSL K6-2865]
MKNIETYSRNIINEAYMARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986143 NYE42_RS12785 WP_342496986.1 2619845..2620915(-) (comGA) [Bacillus sp. FSL K6-2865]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCCACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment