Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHB83_RS02180 Genome accession   NZ_CP152034
Coordinates   409754..410881 (-) Length   375 a.a.
NCBI ID   WP_277551598.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3149     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 404754..415881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB83_RS02145 (MHB83_02145) flgL 404934..405845 (-) 912 WP_137054345.1 flagellar hook-associated protein FlgL -
  MHB83_RS02150 (MHB83_02150) flgK 405855..407375 (-) 1521 WP_253622115.1 flagellar hook-associated protein FlgK -
  MHB83_RS02155 (MHB83_02155) - 407391..407873 (-) 483 WP_050944342.1 flagellar protein FlgN -
  MHB83_RS02160 (MHB83_02160) flgM 407892..408155 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHB83_RS02165 (MHB83_02165) - 408235..408654 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  MHB83_RS02170 (MHB83_02170) comFC 408711..409217 (-) 507 WP_050782676.1 phosphoribosyltransferase family protein Machinery gene
  MHB83_RS02175 (MHB83_02175) - 409394..409687 (-) 294 WP_106066158.1 late competence development ComFB family protein -
  MHB83_RS02180 (MHB83_02180) comFA 409754..410881 (-) 1128 WP_277551598.1 DEAD/DEAH box helicase Machinery gene
  MHB83_RS02185 (MHB83_02185) - 411253..412095 (-) 843 WP_226804196.1 DegV family protein -
  MHB83_RS02190 (MHB83_02190) degU 412313..413002 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHB83_RS02195 (MHB83_02195) degS 413067..414242 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MHB83_RS02200 (MHB83_02200) - 414464..415132 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42884.31 Da        Isoelectric Point: 10.4626

>NTDB_id=985983 MHB83_RS02180 WP_277551598.1 409754..410881(-) (comFA) [Bacillus sp. FSL K6-3149]
MMGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGM
SVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDERLQF
AVLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQKHITK
KRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIF
TESALVQISGRAGRHLDFFKGDVFFFHFGLTRSMKQAMKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=985983 MHB83_RS02180 WP_277551598.1 409754..410881(-) (comFA) [Bacillus sp. FSL K6-3149]
ATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATT
AACATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATC
TACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATG
AGTGTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAA
AATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTCACCAGTTGATGA
GATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAACGTCTCCAATTT
GCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAATGATAAGAGATGT
ATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGA
TTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAA
AAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCATTTAAA
TGTAGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAATACGATGTACTAG
TTACAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTGCAAGTGGGGGTACTAGGTGCAGAATCTACTATCTTT
ACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCTCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAATGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCG
AAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-334)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

58.378

98.667

0.576
  comFA Latilactobacillus sakei subsp. sakei 23K

39.247

99.2

0.389
  comFA/cflA Streptococcus mitis SK321

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae Rx1

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae D39

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae R6

40.113

94.4

0.379
  comFA/cflA Streptococcus pneumoniae TIGR4

39.831

94.4

0.376
  comFA/cflA Streptococcus mitis NCTC 12261

39.548

94.4

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

38.162

95.733

0.365

Multiple sequence alignment