Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHI60_RS15025 Genome accession   NZ_CP152032
Coordinates   2945940..2946233 (+) Length   97 a.a.
NCBI ID   WP_144484628.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3200     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2940940..2951233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI60_RS14990 (MHI60_14990) - 2941034..2941672 (+) 639 WP_144484634.1 MBL fold metallo-hydrolase -
  MHI60_RS14995 (MHI60_14995) - 2941720..2941962 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI60_RS15000 (MHI60_15000) - 2942175..2942555 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI60_RS15010 (MHI60_15010) - 2942779..2943654 (+) 876 WP_144484632.1 STAS domain-containing protein -
  MHI60_RS15015 (MHI60_15015) comGA 2943832..2944902 (+) 1071 WP_111292287.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI60_RS15020 (MHI60_15020) comGB 2944883..2945923 (+) 1041 WP_144484630.1 competence type IV pilus assembly protein ComGB -
  MHI60_RS15025 (MHI60_15025) comGC 2945940..2946233 (+) 294 WP_144484628.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI60_RS15030 (MHI60_15030) comGD 2946226..2946672 (+) 447 WP_144484626.1 competence type IV pilus minor pilin ComGD -
  MHI60_RS15035 (MHI60_15035) comGE 2946656..2946970 (+) 315 WP_144484624.1 competence type IV pilus minor pilin ComGE -
  MHI60_RS15040 (MHI60_15040) comGF 2946882..2947409 (+) 528 WP_186437312.1 competence type IV pilus minor pilin ComGF -
  MHI60_RS15045 (MHI60_15045) comGG 2947406..2947789 (+) 384 WP_144484620.1 competence type IV pilus minor pilin ComGG -
  MHI60_RS15050 (MHI60_15050) - 2947849..2948043 (+) 195 WP_024718846.1 YqzE family protein -
  MHI60_RS15055 (MHI60_15055) - 2948077..2948355 (-) 279 WP_223251017.1 DUF3889 domain-containing protein -
  MHI60_RS15060 (MHI60_15060) tapA 2948655..2949182 (+) 528 WP_095408998.1 amyloid fiber anchoring/assembly protein TapA -
  MHI60_RS15065 (MHI60_15065) - 2949235..2949807 (+) 573 WP_144484618.1 signal peptidase I -
  MHI60_RS15070 (MHI60_15070) - 2949862..2950662 (+) 801 WP_111292280.1 TasA family protein -
  MHI60_RS15075 (MHI60_15075) sinR 2950730..2951065 (-) 336 WP_024422969.1 transcriptional regulator SinR Regulator

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10709.66 Da        Isoelectric Point: 7.3837

>NTDB_id=985906 MHI60_RS15025 WP_144484628.1 2945940..2946233(+) (comGC) [Bacillus sp. FSL K6-3200]
MNDKGFTLIEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=985906 MHI60_RS15025 WP_144484628.1 2945940..2946233(+) (comGC) [Bacillus sp. FSL K6-3200]
ATGAATGATAAAGGATTTACCCTGATAGAAATGTTAATTGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTCAAATCAATGGTCAGTACACAAGTGATGG
CATATGAATTGGAGCATGACGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.391

94.845

0.402
  comGC Staphylococcus aureus N315

42.391

94.845

0.402

Multiple sequence alignment