Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHI23_RS16425 Genome accession   NZ_CP152024
Coordinates   3183008..3184405 (-) Length   465 a.a.
NCBI ID   WP_277600436.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0003     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3178008..3189405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI23_RS16390 (MHI23_16390) flgL 3178209..3179117 (-) 909 WP_034281095.1 flagellar hook-associated protein FlgL -
  MHI23_RS16395 (MHI23_16395) flgK 3179127..3180647 (-) 1521 WP_342492100.1 flagellar hook-associated protein FlgK -
  MHI23_RS16400 (MHI23_16400) - 3180663..3181145 (-) 483 WP_087977062.1 flagellar protein FlgN -
  MHI23_RS16405 (MHI23_16405) flgM 3181164..3181427 (-) 264 WP_024423620.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHI23_RS16410 (MHI23_16410) - 3181507..3181926 (-) 420 WP_024423621.1 TIGR03826 family flagellar region protein -
  MHI23_RS16415 (MHI23_16415) comFC 3181983..3182639 (-) 657 WP_241218534.1 ComF family protein Machinery gene
  MHI23_RS16420 (MHI23_16420) - 3182666..3182959 (-) 294 WP_024423623.1 competence protein ComFB -
  MHI23_RS16425 (MHI23_16425) comFA 3183008..3184405 (-) 1398 WP_277600436.1 DEAD/DEAH box helicase Machinery gene
  MHI23_RS16430 (MHI23_16430) - 3184526..3185368 (-) 843 WP_024423625.1 DegV family protein -
  MHI23_RS16435 (MHI23_16435) degU 3185584..3186273 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHI23_RS16440 (MHI23_16440) degS 3186338..3187513 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MHI23_RS16445 (MHI23_16445) - 3187734..3188402 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53203.09 Da        Isoelectric Point: 9.8999

>NTDB_id=985472 MHI23_RS16425 WP_277600436.1 3183008..3184405(-) (comFA) [Bacillus sp. FSL M7-0003]
MNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKATECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMRVCIATPRTDVVLELEPRLTKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ
RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHSDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=985472 MHI23_RS16425 WP_277600436.1 3183008..3184405(-) (comFA) [Bacillus sp. FSL M7-0003]
TTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGTCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGCAACGGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAGGGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTACAAAAGCATT
TCAAGGGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAGAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTCAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTGAACGTGGAGGGGGTATCTGCCGATGATCCGGATAGAAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACTCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(324-420)

Type III restriction enzyme, res subunit (ResIII)

(116-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.412

98.065

0.514
  comFA/cflA Streptococcus pneumoniae Rx1

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae D39

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae R6

38.095

94.839

0.361

Multiple sequence alignment