Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYE68_RS13445 Genome accession   NZ_CP152012
Coordinates   2709444..2710514 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0447     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704444..2715514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE68_RS13395 (NYE68_13395) - 2704504..2705088 (-) 585 WP_015240205.1 signal peptidase I -
  NYE68_RS13400 (NYE68_13400) tapA 2705060..2705731 (-) 672 WP_324636720.1 amyloid fiber anchoring/assembly protein TapA -
  NYE68_RS13405 (NYE68_13405) - 2705990..2706319 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NYE68_RS13410 (NYE68_13410) - 2706359..2706538 (-) 180 WP_003153093.1 YqzE family protein -
  NYE68_RS13415 (NYE68_13415) comGG 2706595..2706972 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYE68_RS13420 (NYE68_13420) comGF 2706973..2707368 (-) 396 WP_324636718.1 competence type IV pilus minor pilin ComGF -
  NYE68_RS13425 (NYE68_13425) comGE 2707382..2707696 (-) 315 WP_324636717.1 competence type IV pilus minor pilin ComGE -
  NYE68_RS13430 (NYE68_13430) comGD 2707680..2708117 (-) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE68_RS13435 (NYE68_13435) comGC 2708107..2708415 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE68_RS13440 (NYE68_13440) comGB 2708420..2709457 (-) 1038 WP_154817564.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE68_RS13445 (NYE68_13445) comGA 2709444..2710514 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE68_RS13450 (NYE68_13450) - 2710707..2711657 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NYE68_RS13455 (NYE68_13455) - 2711803..2713104 (+) 1302 WP_324636716.1 hemolysin family protein -
  NYE68_RS13460 (NYE68_13460) - 2713142..2713972 (-) 831 WP_015417821.1 STAS domain-containing protein -
  NYE68_RS13470 (NYE68_13470) - 2714179..2714559 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYE68_RS13475 (NYE68_13475) - 2714796..2715041 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=984833 NYE68_RS13445 WP_007408320.1 2709444..2710514(-) (comGA) [Bacillus sp. FSL R5-0447]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=984833 NYE68_RS13445 WP_007408320.1 2709444..2710514(-) (comGA) [Bacillus sp. FSL R5-0447]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGTTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment