Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NST73_RS16665 Genome accession   NZ_CP151989
Coordinates   3219239..3220366 (-) Length   375 a.a.
NCBI ID   WP_235672188.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1034     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3214239..3225366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST73_RS16630 (NST73_16630) flgL 3214422..3215333 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  NST73_RS16635 (NST73_16635) flgK 3215342..3216862 (-) 1521 WP_024719239.1 flagellar hook-associated protein FlgK -
  NST73_RS16640 (NST73_16640) - 3216878..3217360 (-) 483 WP_007499977.1 flagellar protein FlgN -
  NST73_RS16645 (NST73_16645) flgM 3217379..3217642 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NST73_RS16650 (NST73_16650) - 3217722..3218141 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NST73_RS16655 (NST73_16655) - 3218198..3218920 (-) 723 WP_272950036.1 ComF family protein -
  NST73_RS16660 (NST73_16660) - 3218881..3219174 (-) 294 WP_008348267.1 competence protein ComFB -
  NST73_RS16665 (NST73_16665) comFA 3219239..3220366 (-) 1128 WP_235672188.1 DEAD/DEAH box helicase Machinery gene
  NST73_RS16670 (NST73_16670) - 3220738..3221580 (-) 843 WP_024719242.1 DegV family protein -
  NST73_RS16675 (NST73_16675) degU 3221782..3222471 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NST73_RS16680 (NST73_16680) degS 3222536..3223711 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  NST73_RS16685 (NST73_16685) - 3223933..3224601 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42926.95 Da        Isoelectric Point: 10.4270

>NTDB_id=983761 NST73_RS16665 WP_235672188.1 3219239..3220366(-) (comFA) [Bacillus sp. FSL W7-1034]
MLGKLSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAVLYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSIDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVAHRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=983761 NST73_RS16665 WP_235672188.1 3219239..3220366(-) (comFA) [Bacillus sp. FSL W7-1034]
ATGCTGGGAAAATTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCTGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGTACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCGCCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTTGACGAAGTCGATGCATTTCCTTATTCAATTGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCATAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCATTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTATTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-335)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.135

98.667

0.544
  comFA/cflA Streptococcus pneumoniae Rx1

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae D39

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae R6

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae TIGR4

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.441

99.2

0.381
  comFA/cflA Streptococcus mitis NCTC 12261

40.395

94.4

0.381
  comFA/cflA Streptococcus mitis SK321

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.158

97.6

0.363

Multiple sequence alignment