Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NST65_RS11200 Genome accession   NZ_CP151983
Coordinates   2200384..2200677 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus sp. FSL P4-0248     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195384..2205677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST65_RS11150 (NST65_11150) sinR 2195548..2195883 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  NST65_RS11155 (NST65_11155) - 2195951..2196751 (-) 801 WP_034280222.1 TasA family protein -
  NST65_RS11160 (NST65_11160) - 2196809..2197381 (-) 573 WP_034280218.1 signal peptidase I -
  NST65_RS11165 (NST65_11165) tapA 2197434..2197961 (-) 528 WP_065214636.1 amyloid fiber anchoring/assembly protein TapA -
  NST65_RS11170 (NST65_11170) - 2198261..2198539 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  NST65_RS11175 (NST65_11175) - 2198574..2198768 (-) 195 WP_046311750.1 YqzE family protein -
  NST65_RS11180 (NST65_11180) comGG 2198828..2199211 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  NST65_RS11185 (NST65_11185) comGF 2199208..2199735 (-) 528 WP_259407219.1 competence type IV pilus minor pilin ComGF -
  NST65_RS11190 (NST65_11190) comGE 2199647..2199961 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  NST65_RS11195 (NST65_11195) comGD 2199945..2200391 (-) 447 WP_046311754.1 competence type IV pilus minor pilin ComGD -
  NST65_RS11200 (NST65_11200) comGC 2200384..2200677 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  NST65_RS11205 (NST65_11205) comGB 2200694..2201734 (-) 1041 WP_111926463.1 competence type IV pilus assembly protein ComGB -
  NST65_RS11210 (NST65_11210) comGA 2201715..2202785 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  NST65_RS11215 (NST65_11215) - 2202963..2203838 (-) 876 WP_024422979.1 STAS domain-containing protein -
  NST65_RS11225 (NST65_11225) - 2204062..2204442 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST65_RS11230 (NST65_11230) - 2204655..2204897 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NST65_RS11235 (NST65_11235) - 2204943..2205581 (-) 639 WP_111926461.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=983375 NST65_RS11200 WP_024422977.1 2200384..2200677(-) (comGC) [Bacillus sp. FSL P4-0248]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983375 NST65_RS11200 WP_024422977.1 2200384..2200677(-) (comGC) [Bacillus sp. FSL P4-0248]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTTGTAATATCCATTCTTTTACTCATTACCATACC
GAATATTACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAAGGATTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.222

92.784

0.392
  comGC Staphylococcus aureus N315

42.222

92.784

0.392

Multiple sequence alignment