Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACN3VN_RS10820 Genome accession   NZ_CP183396
Coordinates   2345568..2346008 (+) Length   146 a.a.
NCBI ID   WP_058569267.1    Uniprot ID   -
Organism   Xylella fastidiosa strain CFBP8074     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2340568..2351008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN3VN_RS10790 (ACN3VN_10790) coaE 2340669..2341292 (-) 624 WP_046418843.1 dephospho-CoA kinase -
  ACN3VN_RS10795 (ACN3VN_10795) pilD 2341304..2342167 (-) 864 WP_058569265.1 prepilin peptidase Machinery gene
  ACN3VN_RS10800 (ACN3VN_10800) pilC 2342174..2343376 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  ACN3VN_RS10805 (ACN3VN_10805) pilA/pilAI 2343817..2344266 (+) 450 WP_058569266.1 pilin Machinery gene
  ACN3VN_RS10810 (ACN3VN_10810) - 2344531..2344734 (-) 204 WP_419653900.1 hypothetical protein -
  ACN3VN_RS10815 (ACN3VN_10815) - 2345239..2345367 (-) 129 WP_267885865.1 hypothetical protein -
  ACN3VN_RS10820 (ACN3VN_10820) pilA/pilAI 2345568..2346008 (+) 441 WP_058569267.1 pilin Machinery gene
  ACN3VN_RS10825 (ACN3VN_10825) pilB 2346849..2348582 (+) 1734 WP_058569268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN3VN_RS10830 (ACN3VN_10830) - 2348676..2350088 (-) 1413 WP_058569269.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14947.22 Da        Isoelectric Point: 8.1165

>NTDB_id=983205 ACN3VN_RS10820 WP_058569267.1 2345568..2346008(+) (pilA/pilAI) [Xylella fastidiosa strain CFBP8074]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVAAALAEITPGRTQAEIRMADGQASNTPNAIGLRSPTTRCSL
VVVDITAAGGTIICTMVGNGQVNNQTITLTRIADNNAGQGGVNTGGNWTCTTTAPAALTPAGCTGT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=983205 ACN3VN_RS10820 WP_058569267.1 2345568..2346008(+) (pilA/pilAI) [Xylella fastidiosa strain CFBP8074]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATCGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTGGCCAGGTCTCAAGTCGCAGCGGCACTAGCCGAGATCACACCGGGCCGAACACAAGCCG
AAATCCGTATGGCTGATGGACAGGCTTCAAACACTCCTAACGCTATTGGACTACGCAGCCCGACCACTCGTTGCAGCCTC
GTTGTGGTGGACATCACAGCTGCTGGCGGCACAATCATATGTACGATGGTTGGTAATGGTCAGGTCAACAACCAAACTAT
CACCTTGACCCGCATCGCTGATAACAACGCAGGCCAAGGTGGCGTCAATACTGGGGGGAACTGGACCTGTACCACAACCG
CGCCTGCAGCGTTAACTCCAGCAGGCTGCACTGGAACTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.622

100

0.473
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.425
  pilA Vibrio cholerae C6706

40.26

100

0.425
  pilA Vibrio cholerae strain A1552

40.26

100

0.425
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.26

100

0.425
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.466

100

0.425
  pilA Acinetobacter baumannii strain A118

39.865

100

0.404
  pilA Vibrio parahaemolyticus RIMD 2210633

38.621

99.315

0.384
  pilA Haemophilus influenzae Rd KW20

35.811

100

0.363
  comP Acinetobacter baylyi ADP1

33.758

100

0.363

Multiple sequence alignment