Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACN3VN_RS10805 Genome accession   NZ_CP183396
Coordinates   2343817..2344266 (+) Length   149 a.a.
NCBI ID   WP_058569266.1    Uniprot ID   -
Organism   Xylella fastidiosa strain CFBP8074     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338817..2349266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN3VN_RS10785 (ACN3VN_10785) - 2339065..2340402 (+) 1338 WP_023908103.1 sensor histidine kinase -
  ACN3VN_RS10790 (ACN3VN_10790) coaE 2340669..2341292 (-) 624 WP_046418843.1 dephospho-CoA kinase -
  ACN3VN_RS10795 (ACN3VN_10795) pilD 2341304..2342167 (-) 864 WP_058569265.1 prepilin peptidase Machinery gene
  ACN3VN_RS10800 (ACN3VN_10800) pilC 2342174..2343376 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  ACN3VN_RS10805 (ACN3VN_10805) pilA/pilAI 2343817..2344266 (+) 450 WP_058569266.1 pilin Machinery gene
  ACN3VN_RS10810 (ACN3VN_10810) - 2344531..2344734 (-) 204 WP_419653900.1 hypothetical protein -
  ACN3VN_RS10815 (ACN3VN_10815) - 2345239..2345367 (-) 129 WP_267885865.1 hypothetical protein -
  ACN3VN_RS10820 (ACN3VN_10820) pilA/pilAI 2345568..2346008 (+) 441 WP_058569267.1 pilin Machinery gene
  ACN3VN_RS10825 (ACN3VN_10825) pilB 2346849..2348582 (+) 1734 WP_058569268.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15554.72 Da        Isoelectric Point: 8.5006

>NTDB_id=983204 ACN3VN_RS10805 WP_058569266.1 2343817..2344266(+) (pilA/pilAI) [Xylella fastidiosa strain CFBP8074]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQVTAALADITPGKVQAESLIADSKTTDKPADIGLRPDTTRCGI
TVKVDTTGLANITCTIKGNSQVNGQTIAWNRSVDNSAGTNGANNGGQWTCNSTVSSDALRPSGCLASTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=983204 ACN3VN_RS10805 WP_058569266.1 2343817..2344266(+) (pilA/pilAI) [Xylella fastidiosa strain CFBP8074]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTCCTAGCAGCCATCGCGCT
GCCTATGTACCAAAATTATGTCGCCAGATCCCAAGTCACTGCGGCATTAGCCGACATCACCCCGGGCAAGGTGCAAGCCG
AGTCTCTGATTGCTGATTCGAAGACTACAGACAAGCCAGCCGATATCGGACTGCGCCCCGACACCACTCGTTGTGGGATT
ACCGTTAAAGTCGATACCACCGGCCTTGCCAACATCACATGTACGATTAAGGGCAATTCTCAGGTGAATGGTCAAACCAT
CGCTTGGAATCGTAGCGTCGATAACTCCGCGGGCACCAATGGCGCCAATAATGGAGGTCAGTGGACATGCAACTCCACTG
TGTCATCCGATGCATTGAGGCCCTCAGGCTGCCTTGCCTCTACTAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.919

99.329

0.436
  pilA Acinetobacter baumannii strain A118

40.132

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.497

98.658

0.409
  pilA Pseudomonas aeruginosa PAK

36.478

100

0.389
  pilA Vibrio cholerae C6706

39.189

99.329

0.389
  pilA Vibrio cholerae strain A1552

39.189

99.329

0.389
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.189

99.329

0.389
  pilA Vibrio parahaemolyticus RIMD 2210633

38.514

99.329

0.383
  pilA Ralstonia pseudosolanacearum GMI1000

33.333

100

0.362

Multiple sequence alignment