Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MHI30_RS13705 Genome accession   NZ_CP151961
Coordinates   2721542..2722171 (-) Length   209 a.a.
NCBI ID   WP_075752231.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2716542..2727171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS13685 (MHI30_13685) holA 2717101..2718126 (-) 1026 WP_167564576.1 DNA polymerase III subunit delta -
  MHI30_RS13690 (MHI30_13690) - 2718356..2718487 (+) 132 WP_003183686.1 YqzM family protein -
  MHI30_RS13695 (MHI30_13695) - 2718512..2720899 (-) 2388 WP_075752233.1 DNA internalization-related competence protein ComEC/Rec2 -
  MHI30_RS13700 (MHI30_13700) - 2720904..2721473 (-) 570 WP_020452273.1 ComE operon protein 2 -
  MHI30_RS13705 (MHI30_13705) comEA 2721542..2722171 (-) 630 WP_075752231.1 helix-hairpin-helix domain-containing protein Machinery gene
  MHI30_RS13710 (MHI30_13710) comER 2722254..2723075 (+) 822 WP_075752229.1 late competence protein ComER -
  MHI30_RS13715 (MHI30_13715) - 2723145..2723900 (-) 756 WP_020452276.1 class I SAM-dependent methyltransferase -
  MHI30_RS13720 (MHI30_13720) rsfS 2723897..2724253 (-) 357 WP_020452277.1 ribosome silencing factor -
  MHI30_RS13725 (MHI30_13725) yqeK 2724267..2724830 (-) 564 WP_020452278.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MHI30_RS13730 (MHI30_13730) - 2724820..2725389 (-) 570 WP_075752227.1 nicotinate-nucleotide adenylyltransferase -
  MHI30_RS13735 (MHI30_13735) yhbY 2725408..2725698 (-) 291 WP_075752225.1 ribosome assembly RNA-binding protein YhbY -
  MHI30_RS13740 (MHI30_13740) aroE 2725692..2726528 (-) 837 WP_075752223.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22619.51 Da        Isoelectric Point: 4.7085

>NTDB_id=982269 MHI30_RS13705 WP_075752231.1 2721542..2722171(-) (comEA) [Bacillus sp. FSL L8-0528]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSDISIPEEASAQTFDKKEEVKLKKNAGREEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQQQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSGDIVNINTASSEELQT
IPGIGPSKAEAVIEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=982269 MHI30_RS13705 WP_075752231.1 2721542..2722171(-) (comEA) [Bacillus sp. FSL L8-0528]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGAGTGGCACTCGTCTTGATCATCAGCGCGGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGACATTTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAAGTGAAGCTTAAAAAAAACGCGGGGAGAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAACAGCA
AATTAATCTGGCGGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAGGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAATCGAATACCGTGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(146-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.804

100

0.541
  comEA Staphylococcus aureus MW2

35.909

100

0.378
  comEA Staphylococcus aureus N315

35.455

100

0.373

Multiple sequence alignment