Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAFX31_RS14205 Genome accession   NZ_CP151941
Coordinates   1300405..1301430 (-) Length   341 a.a.
NCBI ID   WP_116871578.1    Uniprot ID   -
Organism   Vibrio chagasii strain TBV018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1295405..1306430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX31_RS14180 (AAFX31_14190) aroK 1296264..1296782 (-) 519 WP_004737165.1 shikimate kinase AroK -
  AAFX31_RS14185 (AAFX31_14195) pilQ 1296959..1298677 (-) 1719 WP_105057647.1 type IV pilus secretin PilQ Machinery gene
  AAFX31_RS14190 (AAFX31_14200) pilP 1298713..1299231 (-) 519 WP_061033622.1 pilus assembly protein PilP Machinery gene
  AAFX31_RS14195 (AAFX31_14205) pilO 1299218..1299829 (-) 612 WP_171762284.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFX31_RS14200 (AAFX31_14210) pilN 1299822..1300421 (-) 600 WP_008222290.1 PilN domain-containing protein Machinery gene
  AAFX31_RS14205 (AAFX31_14215) pilM 1300405..1301430 (-) 1026 WP_116871578.1 type IV pilus assembly protein PilM Machinery gene
  AAFX31_RS14210 (AAFX31_14220) - 1301582..1304143 (+) 2562 WP_061033618.1 penicillin-binding protein 1A -
  AAFX31_RS14215 (AAFX31_14225) oxyR 1304235..1305128 (-) 894 WP_004737158.1 DNA-binding transcriptional regulator OxyR -
  AAFX31_RS14220 (AAFX31_14230) - 1305349..1306077 (+) 729 WP_004737157.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38146.61 Da        Isoelectric Point: 6.5746

>NTDB_id=981413 AAFX31_RS14205 WP_116871578.1 1300405..1301430(-) (pilM) [Vibrio chagasii strain TBV018]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VLVDTQAHGLLHVWQLASRLYPEKSNWLLVDIGVDQTSMGIIPQGAAPFYKDIAFGTQDLCSIENPNDIEAAFGSAQDTH
QFIVNLIEKLKRQLQLYSSVNAHQPISGIWLMGEGASTPMVAEELERHFQLSCESLNPLSLFENKVARKRRLQMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=981413 AAFX31_RS14205 WP_116871578.1 1300405..1301430(-) (pilM) [Vibrio chagasii strain TBV018]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCTGTGGTGCTAAAACCCGTGGGGGAATT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTCGGCTGTCGTAATGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAGGTACTGCAAATTGAGAGTGAACTCGAAGACAGAGAGAAAGAGTTTTCGATTTATCAAACCTTCGCCCA
TCAATCTCCTTTTCCCATCGAGGAGCTGAGTTTAGATTTTGTGAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGCTATCAGGTTTATGCCACTCGTAAAGAAGTCGTTGATAGCCGAGCGGATGCATTAACTAAGGCTGGTTTTAAACCT
GTGCTGGTGGATACGCAGGCGCATGGCTTGCTTCATGTGTGGCAGCTTGCCTCACGCTTGTACCCAGAGAAAAGTAATTG
GCTGTTGGTGGACATTGGCGTTGATCAAACCTCGATGGGCATCATTCCTCAGGGAGCAGCCCCTTTCTATAAAGACATCG
CTTTTGGTACGCAAGACCTTTGCAGTATCGAAAACCCCAATGATATCGAGGCTGCGTTTGGCAGTGCTCAGGATACTCAT
CAATTTATCGTTAACTTAATTGAAAAGCTCAAACGTCAGTTGCAGCTCTATTCCTCGGTGAATGCTCACCAACCTATCTC
AGGCATCTGGTTAATGGGCGAGGGCGCCAGCACACCAATGGTGGCTGAAGAGCTTGAACGACATTTTCAGTTGAGCTGTG
AATCATTGAATCCTTTATCCCTGTTTGAGAATAAGGTCGCGAGGAAGCGCCGCCTGCAAATGGATTGGCAACATTTTGGT
ATTGCTGCGGGCATGGCGATGAGCGGACTTAAATGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.545

100

0.545
  pilM Vibrio campbellii strain DS40M4

49.275

100

0.499

Multiple sequence alignment