Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAFX31_RS14185 Genome accession   NZ_CP151941
Coordinates   1296959..1298677 (-) Length   572 a.a.
NCBI ID   WP_105057647.1    Uniprot ID   -
Organism   Vibrio chagasii strain TBV018     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291959..1303677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX31_RS14165 (AAFX31_14175) - 1292647..1293492 (-) 846 WP_004737168.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AAFX31_RS14170 (AAFX31_14180) - 1293562..1295070 (-) 1509 WP_411062189.1 SPOR domain-containing protein -
  AAFX31_RS14175 (AAFX31_14185) aroB 1295150..1296238 (-) 1089 WP_411062190.1 3-dehydroquinate synthase -
  AAFX31_RS14180 (AAFX31_14190) aroK 1296264..1296782 (-) 519 WP_004737165.1 shikimate kinase AroK -
  AAFX31_RS14185 (AAFX31_14195) pilQ 1296959..1298677 (-) 1719 WP_105057647.1 type IV pilus secretin PilQ Machinery gene
  AAFX31_RS14190 (AAFX31_14200) pilP 1298713..1299231 (-) 519 WP_061033622.1 pilus assembly protein PilP Machinery gene
  AAFX31_RS14195 (AAFX31_14205) pilO 1299218..1299829 (-) 612 WP_171762284.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFX31_RS14200 (AAFX31_14210) pilN 1299822..1300421 (-) 600 WP_008222290.1 PilN domain-containing protein Machinery gene
  AAFX31_RS14205 (AAFX31_14215) pilM 1300405..1301430 (-) 1026 WP_116871578.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62123.65 Da        Isoelectric Point: 4.3089

>NTDB_id=981409 AAFX31_RS14185 WP_105057647.1 1296959..1298677(-) (pilQ) [Vibrio chagasii strain TBV018]
MNKGISGLVSKTLQGFAVSVMLVFSVFGYAESSSNKLENIDFRVNKDKDAVIIIELATSSAVVDVQRAQEGLSIELINTE
VDDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATISNDYQYEYDLKGRFIEVVISKPTIEEVTTEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNEFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSAITPYDDDGGSSVIDDYLNVNLAATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPLLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=981409 AAFX31_RS14185 WP_105057647.1 1296959..1298677(-) (pilQ) [Vibrio chagasii strain TBV018]
ATGAATAAAGGAATAAGTGGATTAGTGAGCAAAACTCTACAAGGTTTTGCTGTGTCCGTGATGTTGGTTTTTAGCGTATT
TGGTTACGCAGAGAGCTCGTCTAATAAATTAGAGAACATTGATTTTAGAGTTAACAAGGATAAAGACGCTGTCATCATCA
TTGAGCTAGCAACAAGCAGCGCGGTGGTCGATGTTCAGCGGGCACAAGAAGGCTTGAGCATCGAACTCATCAATACAGAA
GTGGATGATGACAAGCTCTACCTTTTGGACGTTAAAGATTTCGCGACTCTAGTTGAGGGGATTGAAGTATACAAAGAGAC
GCCAAGCACTCGGTTACTGGCGACCATCAGCAATGACTACCAATACGAATATGACCTCAAGGGGCGCTTCATCGAGGTGG
TGATCAGCAAGCCCACGATTGAAGAAGTGACGACAGAGAAAAGCGTACTTGAGAAAGAGGGCAAACTGATATCGATTAAC
TTCCAAGATATCCCTGTCCGAAACGTGCTGCAGTTAATCGCTGACTACAATGAGTTCAACTTGGTGGTATCCGACTCGGT
CGCGGGTAACCTGACATTACGCTTGGATGGCGTCCCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGCTTAG
ATAAGCGCGTCGATGGCAACGTTATCTTGGTCGCTCCCAAAGCGGAACTGGACCTGAGAGAACAGCAAGCGCTCGAAAAA
TCCCGTCTAGAGGAAGAGCTTGGGGAACTTAAATCAGAAATTATCAAGATTAACTTTGCCAAAGCCACCGATATAGCTGA
CATGATTGGTGGTGAAGGGGCGGTGAGCATGTTGTCTGATCGCGGCTCGATTACCATAGATGAACGCACGAACTCTCTGC
TGATTCGCGAGTTGGAAGAGAACATTGCGGTGATACGCGGCATTATTGAGTCGTTAGATATCCCAGTGAAGCAAGTGCAG
ATAGAGGCGCGTATTGTTACCGTCACCGAAGGTAACCTTGATGAACTCGGCGTGCGCTGGGGCGTTTCATCGACCAACGG
AAGCTTCACGGTTGGCGGTTCAATCGAGGGTAATCACCCTTCAGCCATTACACCTTATGACGACGATGGTGGCAGCAGCG
TGATTGATGACTACCTTAACGTGAATTTGGCGGCGACATCGCCAAATGCATCGAGTATTGCTTTTCAGGTGGCCAAGCTT
GGCTCTGATACGCTGCTCGATCTTGAGCTATCTGCACTGCAACAAGAGTCTAAGGCGGAGATCATCTCTAGCCCACGCTT
AATTACCACAAATAAAAAGCCGGCTTACATTGAGCAAGGTACTGAAATTCCTTACTTAGAGTCCTCTTCGAGTGGTGCAA
CGTCCGTCGCATTTAAGAAAGCTGTGTTGAGTTTGAAAGTTACCCCTCAAATCACACCAGACAATCGCTTGGTTCTGGAT
TTGAGTGTGACTCAAGATAGGCCTGGGCAAGTGGTGAAAACTGGAACCGGGGAAGCGGTCGCGATAGATACCCAAAGGAT
AGGTACGCAAGTGCTTGTTAATAATGGTGAAACCGTCGTTCTTGGTGGAATATTTCAACACAGTGTCAGTAGTACAGTCG
ATAAAGTTCCTCTGTTGGGAGACCTTCCTTTATTGGGTGCATTGTTCCGTCGCAGCTACGAAAATGTGGGTAAAAGTGAA
CTGCTTATCTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

81.387

95.804

0.78
  pilQ Vibrio cholerae strain A1552

77.526

100

0.778
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.526

100

0.778
  pilQ Legionella pneumophila strain ERS1305867

42.909

96.154

0.413
  pilQ Legionella pneumophila strain Lp02

42.909

96.154

0.413
  pilQ Pseudomonas aeruginosa PAK

40.215

97.378

0.392
  comQ Acinetobacter baylyi ADP1

38.879

99.825

0.388
  pilQ Acinetobacter baumannii D1279779

37.847

100

0.381
  pilQ Acinetobacter baumannii strain A118

37.847

100

0.381

Multiple sequence alignment