Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WA044_RS11870 Genome accession   NZ_CP151554
Coordinates   2448235..2449305 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain E69     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2443235..2454305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA044_RS11820 (WA044_11820) - 2443295..2443879 (-) 585 WP_012117977.1 signal peptidase I -
  WA044_RS11825 (WA044_11825) tapA 2443851..2444522 (-) 672 WP_058906184.1 amyloid fiber anchoring/assembly protein TapA -
  WA044_RS11830 (WA044_11830) - 2444781..2445110 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  WA044_RS11835 (WA044_11835) - 2445150..2445329 (-) 180 WP_003153093.1 YqzE family protein -
  WA044_RS11840 (WA044_11840) comGG 2445386..2445763 (-) 378 WP_015388005.1 competence type IV pilus minor pilin ComGG Machinery gene
  WA044_RS11845 (WA044_11845) comGF 2445764..2446159 (-) 396 WP_015388004.1 competence type IV pilus minor pilin ComGF -
  WA044_RS11850 (WA044_11850) comGE 2446173..2446487 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  WA044_RS11855 (WA044_11855) comGD 2446471..2446908 (-) 438 WP_058906185.1 competence type IV pilus minor pilin ComGD Machinery gene
  WA044_RS11860 (WA044_11860) comGC 2446898..2447206 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  WA044_RS11865 (WA044_11865) comGB 2447211..2448248 (-) 1038 WP_058906186.1 competence type IV pilus assembly protein ComGB Machinery gene
  WA044_RS11870 (WA044_11870) comGA 2448235..2449305 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  WA044_RS11875 (WA044_11875) - 2449497..2450447 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  WA044_RS11880 (WA044_11880) - 2450593..2451894 (+) 1302 WP_058906187.1 hemolysin family protein -
  WA044_RS11885 (WA044_11885) - 2451932..2452762 (-) 831 WP_032856863.1 STAS domain-containing protein -
  WA044_RS11895 (WA044_11895) - 2452968..2453348 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WA044_RS11900 (WA044_11900) - 2453585..2453830 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=979127 WA044_RS11870 WP_003153083.1 2448235..2449305(-) (comGA) [Bacillus velezensis strain E69]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=979127 WA044_RS11870 WP_003153083.1 2448235..2449305(-) (comGA) [Bacillus velezensis strain E69]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment