Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   R8522_RS11260 Genome accession   NZ_AP026916
Coordinates   2188800..2190098 (+) Length   432 a.a.
NCBI ID   WP_050123028.1    Uniprot ID   A0A0T8VQI5
Organism   Streptococcus pneumoniae strain PZ900700006     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183800..2195098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8522_RS11235 (PC0006_22230) - 2184323..2184610 (-) 288 Protein_2181 transposase -
  R8522_RS11240 - 2184583..2185121 (+) 539 Protein_2182 transposase -
  R8522_RS11245 (PC0006_22250) cysK 2185196..2186116 (-) 921 WP_000029866.1 cysteine synthase A -
  R8522_RS11250 (PC0006_22260) - 2186382..2187728 (+) 1347 WP_001860172.1 IS1380-like element ISSpn5 family transposase -
  R8522_RS11255 (PC0006_22270) - 2188109..2188744 (-) 636 WP_000395739.1 YigZ family protein -
  R8522_RS11260 (PC0006_22280) comFA/cflA 2188800..2190098 (+) 1299 WP_050123028.1 DEAD/DEAH box helicase Machinery gene
  R8522_RS11265 (PC0006_22290) comFC/cflB 2190095..2190757 (+) 663 WP_000649971.1 ComF family protein Machinery gene
  R8522_RS11270 (PC0006_22300) hpf 2190837..2191385 (+) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R8522_RS11275 (PC0006_22310) - 2191521..2193494 (+) 1974 WP_000715109.1 DHH family phosphoesterase -
  R8522_RS11280 (PC0006_22320) rplI 2193491..2193943 (+) 453 WP_000864220.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49572.49 Da        Isoelectric Point: 8.6005

>NTDB_id=97682 R8522_RS11260 WP_050123028.1 2188800..2190098(+) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700006]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLHIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=97682 R8522_RS11260 WP_050123028.1 2188800..2190098(+) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700006]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
ACATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T8VQI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae TIGR4

99.306

100

0.993
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment