Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   R8775_RS10555 Genome accession   NZ_AP026915
Coordinates   2024953..2026251 (-) Length   432 a.a.
NCBI ID   WP_050233596.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700005     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019953..2031251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8775_RS10535 (PC0005_20370) rplI 2021108..2021560 (-) 453 WP_050114792.1 50S ribosomal protein L9 -
  R8775_RS10540 (PC0005_20380) - 2021557..2023530 (-) 1974 WP_050205401.1 DHH family phosphoesterase -
  R8775_RS10545 (PC0005_20390) hpf 2023666..2024214 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R8775_RS10550 (PC0005_20400) comFC/cflB 2024294..2024956 (-) 663 WP_000649964.1 ComF family protein Machinery gene
  R8775_RS10555 (PC0005_20410) comFA/cflA 2024953..2026251 (-) 1299 WP_050233596.1 DEAD/DEAH box helicase Machinery gene
  R8775_RS10560 (PC0005_20420) - 2026307..2026942 (+) 636 WP_000395730.1 YigZ family protein -
  R8775_RS10565 (PC0005_20430) cysK 2027227..2028147 (+) 921 WP_000029866.1 cysteine synthase A -
  R8775_RS10570 - 2028386..2028760 (-) 375 Protein_2048 IS66 family transposase -
  R8775_RS10575 (PC0005_20450) - 2028733..2029041 (+) 309 Protein_2049 transposase -
  R8775_RS10580 (PC0005_20460) tnpB 2029196..2029519 (-) 324 WP_000586641.1 IS66 family insertion sequence element accessory protein TnpB -
  R8775_RS10585 (PC0005_20470) tsf 2029765..2030805 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49604.53 Da        Isoelectric Point: 8.4382

>NTDB_id=97609 R8775_RS10555 WP_050233596.1 2024953..2026251(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700005]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCLDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=97609 R8775_RS10555 WP_050233596.1 2024953..2026251(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700005]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATTAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTTTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.515

99.769

0.384

Multiple sequence alignment