Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilV   Type   Machinery gene
Locus tag   ABWW09_RS04255 Genome accession   NZ_AP031332
Coordinates   760683..761033 (-) Length   116 a.a.
NCBI ID   WP_353300790.1    Uniprot ID   -
Organism   Thermus thermophilus strain TF27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755683..766033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW09_RS04220 (TthTF27_08280) trpS 756522..757535 (+) 1014 WP_018461708.1 tryptophan--tRNA ligase -
  ABWW09_RS04225 (TthTF27_08290) - 757532..758188 (+) 657 WP_353300786.1 chromosome segregation protein ScpA -
  ABWW09_RS04230 (TthTF27_08300) - 758207..758761 (+) 555 WP_353300787.1 Uma2 family endonuclease -
  ABWW09_RS04235 (TthTF27_08310) - 758795..759157 (-) 363 WP_060384345.1 HepT-like ribonuclease domain-containing protein -
  ABWW09_RS04240 (TthTF27_08320) - 759144..759443 (-) 300 WP_008631315.1 nucleotidyltransferase -
  ABWW09_RS04245 (TthTF27_08330) - 759466..760008 (-) 543 WP_353300788.1 Uma2 family endonuclease -
  ABWW09_RS04250 (TthTF27_08340) - 760129..760434 (-) 306 WP_353300789.1 DUF1640 domain-containing protein -
  ABWW09_RS04255 (TthTF27_08350) pilV 760683..761033 (-) 351 WP_353300790.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABWW09_RS04260 (TthTF27_08360) comZ 761264..762937 (-) 1674 WP_353300791.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  ABWW09_RS04265 (TthTF27_08370) pilA/pilA3 762948..763649 (-) 702 WP_014629427.1 PilW family protein Machinery gene
  ABWW09_RS04270 (TthTF27_08380) pilA/pilA2 763646..764227 (-) 582 WP_353300792.1 type II secretion system protein Machinery gene
  ABWW09_RS04275 (TthTF27_08390) pilA/pilA1 764262..764732 (-) 471 WP_165739610.1 GspH/FimT family protein Machinery gene
  ABWW09_RS04280 (TthTF27_08400) - 764898..765878 (+) 981 WP_353300793.1 YpdA family putative bacillithiol disulfide reductase -

Sequence

Protein


Download         Length: 116 a.a.        Molecular weight: 12174.83 Da        Isoelectric Point: 3.9798

>NTDB_id=97602 ABWW09_RS04255 WP_353300790.1 760683..761033(-) (pilV) [Thermus thermophilus strain TF27]
MRNAKGFTLIELLIVIAIIAILAAVLIPNVLNARKRATDSAAQAFGRQVITWLAAADTAGVDVTEVTSCTDELLQAEGAP
AELPNGVAECSIEYDGAGQWTVTVTSDAGNTFTFTY

Nucleotide


Download         Length: 351 bp        

>NTDB_id=97602 ABWW09_RS04255 WP_353300790.1 760683..761033(-) (pilV) [Thermus thermophilus strain TF27]
ATGCGGAACGCGAAAGGTTTCACCCTGATTGAGCTCCTGATCGTCATCGCCATCATCGCCATCCTGGCGGCGGTGCTGAT
CCCGAACGTCTTGAACGCCCGCAAGCGGGCCACCGATAGCGCCGCTCAGGCCTTTGGCCGCCAAGTCATCACCTGGCTCG
CCGCCGCTGACACGGCCGGTGTGGACGTCACTGAGGTCACGAGCTGTACCGACGAGCTCCTTCAAGCGGAAGGGGCTCCC
GCTGAACTCCCCAATGGGGTAGCTGAGTGCTCCATCGAGTACGATGGCGCCGGTCAGTGGACGGTGACGGTCACGTCTGA
TGCCGGTAACACGTTCACCTTCACTTACTGA

Domains

Predicted by InterProScan.

Pilin A4 (PilA4)

(39-114)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.966

100

0.44
  pilA2 Legionella pneumophila str. Paris

41.739

99.138

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.87

99.138

0.405

Multiple sequence alignment