Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   AABM52_RS02000 Genome accession   NZ_CP150919
Coordinates   463462..464526 (+) Length   354 a.a.
NCBI ID   WP_347910154.1    Uniprot ID   -
Organism   Pseudomonas grandcourensis strain DGS24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458462..469526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM52_RS01990 - 459354..460622 (+) 1269 WP_008047852.1 malic enzyme-like NAD(P)-binding protein -
  AABM52_RS01995 - 460804..463248 (-) 2445 WP_347910153.1 penicillin-binding protein 1A -
  AABM52_RS02000 comM 463462..464526 (+) 1065 WP_347910154.1 pilus assembly protein PilM Machinery gene
  AABM52_RS02005 - 464526..465092 (+) 567 WP_347910155.1 PilN domain-containing protein -
  AABM52_RS02010 pilO 465089..465712 (+) 624 WP_347910156.1 type 4a pilus biogenesis protein PilO -
  AABM52_RS02015 - 465709..466236 (+) 528 WP_347910157.1 pilus assembly protein PilP -
  AABM52_RS02020 pilQ 466250..468319 (+) 2070 WP_347910158.1 type IV pilus secretin PilQ Machinery gene
  AABM52_RS02025 aroK 468324..468842 (+) 519 WP_057712350.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37918.32 Da        Isoelectric Point: 4.6439

>NTDB_id=973797 AABM52_RS02000 WP_347910154.1 463462..464526(+) (comM) [Pseudomonas grandcourensis strain DGS24]
MPGLFNKKSNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPIGAVIEKNIAELEGVGSALARALAKARTGLKNVAV
AVAGSAVITKTIEMDAGLSEDEMESQLKIEADQYIPYPLDEVAIDFEVQGTSGRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFELLASRLAVSGEPSTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAQKKGGLPDDYADEVLQPFREALVQQVSRSLHFFFASGQYSAVDHILLAGGTATIPGLDRLIEQQLGTPTQVANP
FCEMTLGSKVNGAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=973797 AABM52_RS02000 WP_347910154.1 463462..464526(+) (comM) [Pseudomonas grandcourensis strain DGS24]
GTGCCAGGACTCTTCAACAAAAAATCCAATACGCTGCTGGGCATCGATATCAGCTCCACCTCGGTGAAACTTCTTGAGTT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCGTATGCGGTCGAGCCGTTGCCGATCGGTGCGGTGATCGAAAAGAATA
TCGCCGAGCTCGAAGGCGTGGGGTCGGCCCTTGCGCGTGCGCTGGCCAAGGCCAGAACCGGCCTCAAGAACGTGGCCGTG
GCCGTGGCAGGCTCGGCGGTGATCACCAAGACCATCGAAATGGATGCCGGACTTTCCGAGGACGAGATGGAAAGCCAGCT
GAAGATCGAGGCCGATCAGTACATTCCTTATCCCCTGGACGAAGTCGCGATCGACTTTGAAGTCCAGGGCACCTCTGGGC
GCAATCCCGAGCGCGTCAACGTGCTGCTCGCCGCTTGTCGCAAGGAAAACGTCGAAGTCCGCGAGGCAGCCCTGGCCCTG
GCGGGGCTGACCGCCCGGGTGGTCGATGTCGAGGCCTACGCGCTGGAGCGTTCGTTCGAATTACTGGCCAGTCGACTCGC
GGTTTCCGGGGAGCCCTCGACGGTCGCCGTGGTGGACATCGGCGCCACCATGACGACCCTGAGCGTGCTGCACAACGGGC
GGATTATCTACACCCGCGAACAACTGTTCGGCGGTCGGCAGTTGACCGAGGAAATCCAGCGTCGCTATGGCCTGACGCTG
GAGCAGGCGGGGCTGGCGCAAAAGAAGGGCGGTTTGCCGGATGACTATGCCGATGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCGTTGCATTTCTTCTTTGCCTCCGGTCAGTACAGCGCCGTCGACCATATCTTGTTGG
CCGGCGGTACGGCGACGATCCCGGGGCTGGACCGTTTGATCGAGCAGCAGTTGGGCACGCCGACCCAGGTCGCGAACCCT
TTTTGCGAAATGACTCTGGGCAGCAAGGTCAATGGTGCTGCCCTGGCGAGTGATGCCCCGGCGTTGATGATCGCCTGCGG
TCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment